Enzymes
General Enzymes –35 backroom dd 5-2-2002 AvdF
Data Sheets are in the black binder on top of freezer
All New England Biolab unless indicated otherwise.
Restriction Enzymes
AatII GACGT/C 4 37 R0117S
AluI AG/CT 2 37 R0137S
BamH1 G/GATCC BamH1 /BSA 37 R0136L
BbsI GAAGAC(N2)/ 2 37 R0539S
BglI GCCNNNN/NGGC 3 37 R0143S
BglII A/GATCT 3 37 R0144S
BlpI GC/TNAGC 4 37 R0585S
BstEII G/GTNACC 3 60 R0162S
Bsu36I CC/TNAGG 3/BSA 37 R0524S
ClaI AT/CGAT dam 4/BSA 37 R0197S
DpnI GA/TC 4 37 R0176S
DraIII CACNNN/GTG 3/BSA 37 R0510S
EcoRI G/AATTC EcoRI 37 R0101L
EcoRV GAT/ATC 3/BSA 37 R0195S
HincII GTPy/PuAC 3/BSA 37 R0103S
HindIII A/AGCTT 2 37 R0104L
KpnI GGTAC/C 1/BSA 37 R0142S
NcoI C/CATGG 4 37 R0193S
NdeI CA/TATG 4 37 R0111S
NheI G/CTAGC 2/BSA 37 R0131S
NotI GC/GGCCGC 3/BSA 37 R0189L
PstI CTGCA/G 3/BSA 37 R0140S
PvuI CGAT/CG 3/BSA 37 R0150S
PvuII CAG/CTG 2 37 R0151S
SacI GAGCT/C 1/BSA 37 R0156S
SacII CCGC/GG 4 37 R0157S
SalI G/TCGAC Sal/BSA 37 R0138S
ScaI AGT/ACT Sca 37 R0122S
SmaI CCC/GGG 4 25 R0141S
SnaBI TAC/GTA 4/BSA 37 R0130S
SpeI A/CTAGT 2/BSA 37 R0133S
SwaI ATTT/AAAT 3/BSA 25 R0604S
XbaI T/CTAGA 2/BSA 37 R0145L
XhoI C/TCGAG 2/BSA 37 R0146L
XmaI C/CCGGG 4/BSA 37 R0180S
XmnI GAANN/NNTTC 2/BSA 37 R0194S
Miscellaneous Enzymes (5-2-2002 AvdF)
Mung Bean nuclease Mung Bean Buf M0250S
T4 DNA Ligase T4 lig buf M0202L
T4 polynucleotide kinase T4 polynucl buf M0201S
Polymerases
Deep VentR DNA Polymerase ThermoPol buf M0258L
DNA Polymerase I (E coli) EcolPol M0209L
DNA polymerase I Large fragment (Klenow) EcoPol buf M0210S
T4 DNA Polymerase T4 Pol Buf M0203S
TakaRa Taq (Takara R001B)
HotStar Taq (Qiagen 203205)
ProofStar DNA Polymerase (proofread PCR) (Qiagen 202203)
PWO DNA polymerase (Roche 1-644955)
Platinum DNA Polymerase (Life-Gibco 11304-11)
RNA Work
SuperscriptII RNAseH- reverse transcriptase 5x 1rst strand buffer (Invitrogen 18064-014)
T3-RNA Polymerase transcription buf, DTT (Promega P2083)
T7 RNA Polymerase, transcription buff, DTT (Promega, P2075)
SP6 RNA Polymerase transcrip[tion mbuffer, DTT(Promega P1085)
RNAsin (Promega N211B)
BP Ladders
100 bp ladder Amersham 27 4001-01
Hyperladder I Bioline Bio-33026
(PhiX HaeIII 302-6L)
(pBR322 MspI 303-11)
Protein MW markers
Kaleidoscope prestained markers (Biorad 161-0324)