Publications 2008 - 2013: Gerhard Wagner

  1. Z.-Y. J. Sun, K. J. Oh,  M. Kim,  M. Adamowicz, J. West, J. Yu, V. Brusic, Z. Qiao,  J.-h. Wang, G. Wagner, E. L. Reinherz: HIV-1 broadly neutralizing antibody extracts its epitope from a kinked MPER segment on the viral membrane, Immunity, 28, 52-63 (January 2008).

  2. D. P. Frueh, H. Arthanari, A. Koglin, D. Vosburg, A. E. Bennett, C. T. Walsh, and G. Wagner, Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase. Nature, 454, 903-906 (2008), PMC2597408.

  3. A. Koglin, F. Löhr, F. Bernhard, V. Rogov, D. P. Frueh, P. Güntert, G. Wagner, C. T. Walsh, M. A. Marahiel and V. Dötsch: Structural basis for the selectivity of the external thioesterase of the surfactin synthetase. Nature, 454, 907-911 (2008). PMC2854587.

  4. S. Kang, J. Kang, H. Kwon, D. Frueh, S. Hyun Yoo, G. Wagner, and S. Park: Effects of redox potential and Ca2+ on the inositol 1,4,5-trisphosphate receptor L3-1 loop region: implications for receptor regulation, J.Biol. Chem., 283, 25567-25575 (2008). PMC Journal - PMCID In Process.

  5. C. Suzuki, R. Garces, K. Edmonds, S. Hiller, S. Hyberts, A. Marintchev, G. Wagner: PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains.  PNAS 105,3274-3279 (2008). PMC2265113

  6. J. K. Thakur, H. Arthanari, F. Yang, S.-J. Pan, X. Fan, J. Breger, D. P. Frueh, K. Gulshan, D. Li, E. Mylonakis, K. Struhl, W. S. Moye-Rowley, B. P. Cormack, G. Wagner, A. M. Näär: A Nuclear Receptor-Like Pathway Regulating Multidrug Resistance in Fungi. Nature, 452, 604-609 (2008).

  7. P. Selenko, D. P. Frueh, S. J. Elsaesser, W. Haas, S. P. Gygi, G. Wagner: In situ observation of protein phosphorylation by high-resolution NMR spectroscopy. NSMB, 15, 321-329 (2008).

  8. A. Catic, Z.-Y. J Sun, D.M Ratner, S. Misaghi, E. Spooner, J. Samuelson, G. Wagner, H.L Ploegh: Sequence and structure evolved separately in a ribosomal ubiquitin variant. EMBO J. 26, 3474 - 3483 (2007).

  9. K. Takeuchi; H. Yang; E. Ng; S. Park; Z.-Y. J. Sun; Ellis L Reinherz; Gerhard Wagner: Structure of the CD3e-Nck complex and its role in TCR signaling and internalization. J. Mol. Biol., 380,704-716 (2008), PMC2577852.

  10. G. Wagner:  Introduction: NMR Resonance in Undergraduate Education.   Chapter 1, Modern NMR Spectroscopy in Education. D. Rovnyak, R. Stockland Jr. (eds.) American Chemical Society,Washington DC, (2007). 

  11. Moerke N.J., Fahmy A., Degterev A., Harbinski F., Gross J.D., Aktas H., Halperin J.A., Wagner G. “Identification of small molecule inhibitors of eIF4E complex formation”, Clinical Cancer Research 9 (16): 6170S-6170S Part 2 Suppl. S, Dec. 2003.

  12. H. Arthanari, D. Frueh, G. Wagner, B. Pryor, N. Khaneja: Fourier synthesis techniques for NMR spectroscopy in iomogenous fields. J. Chem. Phys, 128, 21503 (2008). PMC Journal - PMCID In Process.

  13. L. Lindqvist, M. Oberer, M. Reibarkh, R. Cencic, M.-E. Bordeleau, E. Vogt, A. Marintchev, J. Tanaka, F. Fagotto, M. Altmann, G. Wagner, J. Pelletier: Selective Pharmacological Targeting of a DEAD box RNA helicase, PLoS ONE, 3, e1583 (2008). PMC2216682

  14. Degterev, J. Hitomi, M. Germscheid, I. Ch’en, O. Korkina, X. Teng, D. Abbott, G. D. Cuny, C. Yuan, G. Wagner, S. M. Hedrick, S. A. Gerber, A. Lugovskoy & J. Yuan: Identification Of Rip1 Kinase As A Specific Cellular Target Of Necrostatins. Nature Chem. Biol., 4, 313-321 (2008). PMC: Accepted for publication Mar. 6, 2008.

  15. S. Hiller, R. G. Garces, T. J. Malia, V. Y. Orekhov, M. Colombini, G. Wagner: Solution structure of the integral human membrane protein VDAC-1 in detergent micelles, Science 321, 1206- 1210 (2008). PMC2579273.

  16. Marintchev, K. A. Edmonds, B. Marintcheva, E. Hendrickson, M. Oberer, C. Suzuki, B. Herdy, N. Sonenberg, G. Wagner: The topology of the human eIF4A/4G/4H complex and the regulation of helicase activity, Cell, 136, 447-460. (2009), PMC2656774.

  17. M. Hemond, T. L. Rothstein, G. Wagner: Fas Apoptosis Inhibitory Molecule (FAIM) contains a novel beta sandwich in contact with a partially ordered domain J. Mol. Biol., 386, 1024-1037 (2009). DOI: 10.1016/j.jmb.2009.01.004. PMC274528

  18. S. T. Kim, K. Takeuchi, Z.-Y. J. Sun, M. Touma, C. E. Castro, A. Fahmy, V. P. Dave, D. J. Kappes, M. J. Lang, G. Wagner, E. L. Reinherz: The ab T cell receptor is an anisotropic mechanosensor J. Biol.Chem. 284, 31028-31037 (2009).   PMC2781503

  19. M. Billeter, G. Wagner, K. Wüthrich: Solution NMR structure determination of proteins revisited, J. Biomol. NMR, 42, 155-158 (2008), PMC2748914

  20. K. Takeuchi, Z.-Y. J. Sun, G. Wagner: Alternate 13C-12C labeling for complete mainchain resonance assignments using C alpha direct-detection with applicability toward fast relaxing protein systems. J. Am. Chem. Soc., 130, 17210-17211 (2008), PMC2626166

  21. J. K. Thakur, H. Arthanari, F. Yang, G. Wagner, A. M. Näär: Mediator subunit Gal11p/MED15 is required for fatty acid-dependent gene activation by yeast nuclear receptor-like transcription factor Oaf1p, JBC, 284, 4422-4428 (2009). PMC Journal – Free Article.

  22. Y. Yoffe, M. Léger, A. Zinoviev, J. Zuberek, E. Darzynkiewicz, G. Wagner, M. Shapira: Evolutionary adaptation of the Leishmania eIF4F complex to cap-4 binding involves changes in the eIF4E-eIF4G interactions, Nucleic Acid Res, 37, 3243-3253, (2009) Epub 2009 Mar 24,  PMC2691829

  23. L. Song, Z-Y. J. Sun, K. E. Coleman, M. Zwick, J. Gach, J.-H. Wang, E. L. Reinherz, G. Wagner, M. Kim: Broadly neutralizing anit-HIV-1 antibodies disrupt a hinge-related function of gp41 at the membrane interface. PNAS, 22, 9057-9062, (2009), PMC2690059

  24. S. Hiller, H. Arthanari, G. Wagner: The T-lock: Automated compensation of radio-frequency induced sample heating. J.Biomol.NMR, 44 (2) 69-76 (2009).  PMC2733869

  25. S. Hiller, G.Wagner: The role of solution NMR in the structure determinations of VDAC-1 and other membrane proteins. Curr. Opin. Struct. Biol., 19, 396-401 (2009). PMCID2739811

  26. S. Hiller, I. Ibragimov, G. Wagner, V. Orekhov: Complete decomposition of four-dimensional NOESY spectra, J Am Chem Soc. 131, 12970-12978 (2009). PMC2771277

  27. S. G. Hyberts, D. P. Frueh, H. Arthanari, G. Wagner: FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation, J. Biomol. NMR, 45, 283-294 (2009). PMC2858293

  28. D. P. Frueh, H. Arthanari, A. Koglin, C.T. Walsh, G. Wagner: A double TROSY hNCAnH experiment for efficient assignment of large and challenging proteins, J. Am. Chem. Soc., 131, 12880-12881 (2009).  PMC2749991

  29. D. P. Frueh, A. Leed,  H. Arthanari, A. Koglin,  C. T. Walsh, G. Wagner: Time-shared HSQC-NOESY for accurate distance constraints measured at high-field in 15N-13C-ILV methyl labeled proteins, J. Biomol. NMR, 45, 311-318 (2009). PMC2778061

  30. T. Raschle, S. Hiller, T.Y. Yu, A.J. Rice, T. Walz, G. Wagner: Structural and functional characterization of the integral membrane protein VDAC-1 in lipid bilayer nanodiscs. J Am Chem Soc. 131,17777-17779 (2009). PMC2793270

  31. Wagner, G.: A topical issue: production and labeling of biological macromolecules for NMR investigations. J Biomol NMR 46, 1–2 (2010). Editorial.

  32. Zhou P, Wagner: Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies. J Biomol NMR 46, 23–31 (2010). PMC2879018

  33. Takeuchi, K, Frueh, DP, Hyberts, SG, Sun, Z-Y, Wagner, G: High-resolution 3D CANCA NMR experiments for complete mainchain assignments using Ca direct-detection. J Am Chem Soc., 132, 2945-2951 (2010). PMC2832079

  34. S.G. Hyberts, K. Takeuchi, G. Wagner: Poisson-Gap Sampling and FM Reconstruction for Enhancing Resolution and Sensitivity of Protein NMR Data. J Am Chem Soc., 132, 2945-2951 (2010). PMC2825045

  35. Y. Yu, A. Marintchev, V.G. Kolupaeva,  A. Unbehaun, T. Veryasova, S.C. Lai, P. Hong, G. Wagner, C.U.Hellen, T.V. Pestova: Position of eukaryotic translation initiation factor eIF1A on the 40S ribosomal subunit mapped by directed hydroxyl radical probing. Nucleic Acid Res. 37, 5176-5182 (2009). PMC2731904

  36. S. Hiller, J. Abramson, C. Mannella, G. Wagner, K. Zeth: The 3D structures of VDAC represent a native conformation. Trends Biochem. Sci., 35, 514-521 (2010). PMC2933295

  37. K. Takeuchi, D. P. Frueh,  Z.-Yu J. Sun, S. Hiller, G. Wagner: CACA-TOCSY with alternate 13C-12C labeling: a 13C direct detection experiment for mainchain resonance assignment, dihedral angle information, and amino acid type identification. J. Biomol. NMR, 47, 55-63 (2010). PMC2868269

  38. K. Takeuchi, Z.-Y. Sun, S. Park, G. Wagner: Auto-inhibitory interaction in multidomain adaptor protein Nck: possible roles in improving specificity and functional diversity, Biochemistry, 49, 5634-5641 (2010). PMC2899887

  39. K. Takeuchi, G. Heffron,  Z.-Yu J. Sun, D.P. Frueh, G. Wagner: Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments. J. Biomol. NMR, 47, 271-282 (2010). PMC2946331

  40. S. Hiller, G. Wagner: Solution NMR Spectroscopy of Integral Membrane Proteins. Comprehensive Biophysics, Chapter 5.8: Solution NMR of Integral Membrane Proteins,
    In: Edward H. Egelman, editor: Comprehensive Biophysics, Vol 5, Membranes, Lukas Tamm. Oxford: Academic Press, 2012. pp. 120-138.

  41. S.T. Kim, M. Touma, K. Takeuchi, Z.-Y. Sun, V.P. Dave, D. J. Kappes, G. Wagner, E. L. Reinherz: Distinctive CD3 Heterodimeric Ectodomain Topologies Maximize Antigen-triggered Activation of αβ T Cell Receptors, J. Immunology, 185, 2951-2959 (2010). PMC2936104

  42. M. G. Van der Heiden, J. W. Locasale, K. D. Swanson, H. Sharfi, G.J. Heffron, D. Amador-Noguez, H. R. Christofk, G. Wagner, J. D. Rabinowitz,  J. M. Asara, L. C. Cantley: Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science, 329 1492-1499 (2010). PMC3030121.

  43. S. Kang, H. Kwon, H. Wen, Y. Song, D. Frueh, H.-C. Ahn, S. H. Yoo, G. Wagner, S. Park: Global dynamic conformational changes in the suppressor domain of IP3 receptor by stepwise binding of the two lobes of calmodulin. FASEB J., 25, 840-850 (2011). PMC Journal - In Process.

  44. F. Shaw, H. Arthanari, M. Narovlyansky, A. Durazo, D. P. Frueh, M. P. Pollastri, A. Lee, B. Bilgicer, S. P. Gygi, G. Wagner, G. M. Whitesides: Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability. J. Amer. Chem. Soc., 132,17411-17425 (2010) PMC3135700

  45. K. Takeuchi, M. Gal, H. Takahashi, I. Shimada, G. Wagner: HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment. J. Biomol. NMR, 49, 17-26 (2011). PMC3072286

  46. J.-S. Li, J. Ruths, T.-Y. Yu, H. Arthanari, G. Wagner: Optimal pulse design in quantum control: A unified computational method. PNAS, 108, 1879-1884 (2011) PMC Journal – Free Article.

  47. G. Milbradt, M. Kulkarni, T. Yi, K. Takeuchi, Z.-Y. J. Sun, R. E. Luna, P. Selenko, A. M. Näär, G. Wagner: Structure of the VP16 Transactivator Target in ARC/Mediator, Nature Str. & Mol. Biology, 18, 410-415 (2011). PMC3076674

  48. Arthanari, G. Wagner, N. Khaneja: Heteronuclear Decoupling by Multiple Rotating Frame Technique”, J. Magn. Reson., 209, 8-18 (2011). PMC3101322

  49. S. G. Hyberts, H. Arthanari, G. Wagner, "Wagner, G:  Applications of non-uniform sampling and processing. Top. Curr. Chem., 316, 125-148 (2012).

  50. T.  Raschle,  S. Hiller,  M. Etzkorn,  G. Wagner  Nonmicellar systems for solution NMR spectroscopy of membrane proteins. Curr Opin Struct Biol. 20, 471-479 (2010) PMC2928847
    Miaoa, I. Skidan, J. Yang, A. Lugovskoy, M. Reibarkh, K.Long, T. Brazell, K.A. Durugkar, J. Maki, C. V. Ramana, B. Schaffhausen, G.Wagner, V. Torchilin, J.Yuan, A. Degterev: Small molecule inhibition of phosphatidylinositol-3,4,5-triphosphate (PIP3) binding to pleckstrin homology domains. PNAS, 107, 20126-20131 (2010). PMC2993381

  51. A. Hoeffer, K. K. Cowansage, E.C. Arnold, J. L Banko, N. J. Moerke, R. Rodriguez, E. K. Schmidt, E. Klosi , M. Chorev, R. E. Lloyd, P. Pierre, G. Wagner, J. E. LeDoux, E. Klann: Inhibition of the interactions between eukaryotic initiation factors 4E and 4G impairs long-term associative memory consolidation but not reconsolidation. PNAS, 108, 3383-3388 (2011). PMC3044415

  52. Zinoviev, M. Léger, G. Wagner, M. Shapira: A novel 4E-interacting protein in Leishmania is involved in stage-specific translation pathways. Nucleic Acid Res, 39, 8404-8415 (2011). PMC Journal – Free Access.

  53. Yi, I.-H. Jang, H. Pan, J. Seebacher, S. G. Hyberts, G. J. Heffron, M.G. Vander Heiden, R. Yang, F. Li, J. W. Locasale, H. Sharfi, B. Zhai, R. Rodriguez-Mias, H. Luithardt, L. C. Cantley, G. Q. Daley, J. M. Asara, S.P. Gygi, G. Wagner, C.-F. Liu, J. Yuan: Metabolic regulation of protein N-alpha-acetylation by Bcl-xL promotes cell survival, Cell, 146, 607-620 (2011).  PMC3182480

  54. Fahmy, G. Wagner: Optimization of van Der Waals energy for protein side-chain placement and design. Biophysical J., 101, 1690-1698 (2011).  (PMC Journal – In Process)

  55. B.F. Shaw, G. Schneider, H. Arthanari, M. Narovlyansky, D. Moustakas, A. Durazo, G. Wagner, G. Whitesides: Complexes of native Ubiquitin and dodecyl sulfate illustrate the nature of hydrophobic and electrostatic interactions in the binding of proteins and surfactants. JACS, October 13 (2011). [Epub ahead of print] PMID: 21939262

  56. Kim, Z.-Y.J Sun, K. D. Rand, X. Shi, L. Song, Y. Cheng, A. F. Fahmy, S. Majumdar, G. Ofek, Y. Yang, P. D. Kwong, J.-H. Wang, J. R Engen, G. Wagner, E.L Reinherz: Antibody mechanics on a membrane-bound HIV segment essential for GP41-targeted viral neutralization. Nature Str. & Mol. Biology, 18, 1235-1234 (2011).

  57. S.  Narayanan, H. Arthanari, M. Wolfe, G. Wagner: Molecular characterization of disrupted in schizophrenia-1 risk variant S704C reveals the formation of altered oligomeric assembly. J. Biol. Chem., (2011). published October 13, 2011 as doi:10.1074/jbc.M111.271593

  58. Gal, K. A. Edmonds, A. G. Milbradt, K. Takeuchi, G. Wagner: Speeding up 15N detection: hCaN 2D NMR experiment. J. Biomol. NMR, 51, 497-504 (2011).

  59. B.H. Aktas, J.A. Halperin, G. Wagner, M. Chorev: Inhibition of Translation Initiation as a Novel Paradigm for Cancer Therapy. Ann. Rep. in Med. Chem. 46, 189-210 (2011).

  60. S. T. Kim, Y. Shin, K. Brazin, R. J. Mallis, J. Sun, G. Wagner, M.W. Lang, E. L Reinherz: TCR mechanobiology: Torques and tunable structures linked to early T cell signaling. Frontiers in T Cell Biology, in press.

  61. S.G. Hyberts, A.G. Milbradt, A.B. Wagner, H. Arthanari, G. Wagner: Application of iterative soft thresholding for fast reconstruction of NMR data non-uniformly sampled with multidimensional Poisson Gap scheduling. J. Biomol. NMR, 52, 315-27 (2012).

  62. R.E. Luna, H. Arthanari , H. Hiraishi, J. Nanda, P. Martin-Marcos, M. A. Markus, B. Akabayov, A. G. Milbradt, L. E. Luna, H.-C. Seo, S. G. Hyberts, A. Farmy, M. Reibarkh, D. Miles, P. R. Hagner, E. M. O’Day, T. Yi, A. Marintchev, A. Hinnebusch, J. Lorsch, K. Asano, G. Wagner: C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2. Cell Reports, in press. NIHMSID # 373329

  63. K. Takeuchi, M. Gal, I. Shimada, G. Wagner: Low  detection experiments for biomolecular NMR. In “Recent developments in Biomolecular NMR” . M.C. Clore, J. Potts, editors, in press.

  64. T.-Y. Yu, T. Raschle, S. Hiller, G. Wagner: Solution NMR Spectroscopic Characterization of Human VDAC-2 in Detergent Micelles and Lipid Bilayer Nanodiscs. Biochim Biophys Acta. 1818,1562-1569 (2012). PMC3321126

  65. M.T. Eddy, T.C. Ong, L. Clark, O. Teijido, P.C. van der Wel, R. Garces, G. Wagner, T.K. Rostovtseva, R.G. Griffin: Lipid dynamics and protein-lipid interactions in 2D crystals formed with the ?-barrel integral membrane protein VDAC1. J. Am. Chem. Soc. 134, 6375-6387 (2012). PMC333839.

  66. L. Chen, B.H. Aktas, Y. Wang, X. He, R. Sahoo, N. Zhang, S. Denoyelle, E. Kabha, H. Yang, R.Y. Freedman, J.G. Supko, M. Chorev, G. Wagner, J.A. Halperin: Tumor suppression by small molecule inhibitors of translation initiation. Oncotarget 3, 869-881 (2012). Free Journal.

  67. J.C. Schmidt, H. Arthanari, A. Boeszoermenyi, N. M. Dashkevich, E. M. Wilson-Kubalek, N. Monnier, M. Markus, M. Oberer, R. A. Milligan, M. Bathe5, G. Wagner, E.a L. Grishchuk, I. M. Cheeseman: The kinetochore-bound Ska1 complex tracks depolymerizing microtubules by binding to curved protofilaments. Develop. Cell, Nov 13;23(5):968-80. doi: 10.1016/j.devcel.2012.09.012. Epub 2012 Oct 18. (2012)

  68. P. Coote, H. Arthanari, T. Yu, A. Natarajan, G. Wagner, N. Khaneja: Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames. J. Biomol. NMR, 55, 291-302 (2013) Feb 19. [Epub ahead of print]

  69. S. G. Hyberts,  S.A. Robson, G. Wagner: Exploring signal-to-noise ratio and sensitivity in non-uniformly sampled multi-dimensional NMR spectra. J Biomol NMR. 55, 167-78 (2013) PMC3570699

  70. A. J. Wommack, S. A. Robson, Y. A. Wanniarahchi, A. Wan, C. J. Turner, G. Wagner, E. M. Nolan. NMR Solution Structure Determination and Analytical Ultracentrifugation Studies of the Antimicrobial Peptide Human Defensin. Biochemistry, 51, 9624-9634 (2012) PMC3579768

  71. F. Hagn, M. Etzkorn, T. Raschle, G. Wagner: Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins, JACS, 135, 1919-1925 (2013).  PMC3566289

  72. M. Etzkorn, T. Raschle, F. Hagn, V. Gelev, A.T. Rice, T. Walz, G. Wagner: Cell-Free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility, Structure, 21, 394-401 (2013doi:pii: S0969-2126(13)00009-9. 10.1016/j.str.2013.01.005. [Epub ahead of print] PMID: 23415558, PMC3595385.

  73. S. Akabyov, G. Wagner: Backbone resonance assignment of the HEAT1-domain of the human eukaryotic translation initiation factor 4GI, J. Biomolec. Assignments, 2013 Jan 17. [Epub ahead of print] PMID: 23325513, PMC3640741

  74. B. Akabayov, S. R. Akabayov, S.–J. Lee, G. Wagner, C. C. Richardson: Impact of macromolecular crowding on DNA replication. Nature Communications  4, 1615, (2013). doi: 10.1038/ncomms2620.  NIHMSID 448127

  75. T. Yi, E. Papadopoulos, G. Wagner: HIF-1α promotes cap-dependent translation of selective mRNAs through upregulating eIF4E1 in breast cancer cells under hypoxia conditions. J. Biol. Chem.,  288, 18732-42, (2013)  PMC3696647



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