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Carbohydrate Synthesis/Protein Expression Core (D)

Carbohydrate
Compounds and
Glycan-binding
Proteins

Overview

This Core is producing a library of oligosaccharides for use by Participating Investigators and other Cores. These compounds represent a diverse range of natural oligosaccharide sequences of glycoproteins and glycolipids with linker arms compatible for use as monovalent oligosaccharides and for coupling to biotin, polymers or column supports. This Core is working with the Protein-Carbohydrate Interaction Core (H) to create a glycan array, a library of oligosaccharides for the rapid screening of the carbohydrate binding specificity of glycan-binding proteins (GBPs). This Core is also producing recombinant proteins including glycosyltransferases and GBPs. Information relating to the production of each material, including novel synthetic procedures, is being deposited in the Central Database. Available materials are posted on the website and are distributed to investigators both within and outside of the Consortium upon approval by the Steering Committee.

Specific Objectives

Produce a diverse range of natural oligosaccharide sequences of glycoproteins and glycolipids with linker arms compatible for use as monovalent oligosaccharides and for coupling to proteins or solid supports
Work with the Protein-Carbohydrate Interaction Core (H) to create a glycan array of oligosaccharides for rapid screening of the binding specificity of GBPs
Conduct custom synthesis of oligosaccharides by chemoenzymatic or one pot chemical synthesis methods
Construct multivalent oligosaccharide probes labeled with biotin or fluorescein or other labels for detecting cell surface GBPs
Produce recombinant glycosyltransferases and other enzymes for carbohydrate synthesis
Produce recombinant GBPs for determination of binding specificity by the Protein-Carbohydrate Interaction Core (H), crystallography, and production of antibodies

Core D Team

Jim Paulson and Chi-Huey Wong, Coordinators

Ola Blixt, Director

Nicolai Bovin, Consultant, Compound Library

Nahid Razi, Associate Director, Molecular Biology and Protein Expression

Nathan Jacobsen, Chemical and Enzymatic Synthesis

Daniela Vasiliu, Chemical and Enzymatic Synthesis

Kirk Allen, Protein Expression and Purification

Xiaofei Lui, Vector and Cell Line Construction, Protein Expression and Purification

Yingning Zhang, Vector and Cell Line Construction, Protein Expression and Purification

Last Updated Wednesday, 25-Feb-2004 19:42:40 EST. Please contact us with comments/questions.

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