Antibodies

Protein: Sheep anti-mSiglec F

Type: IgG

Buffer: PBS + 0.1% BSA + 10 mM azide

Purity: IgG was purified from immune serum using protein G Sepharose and affinity-purified using a mSiglec-F-Fc Sepharose CL-4B affinity column. The affinity-purified antibody has been screened against a panel of mouse CD33-related siglecs and found to have no detectable cross-reactivity at up to 10 micrograms/ml.

Storage temperature: 4ºC

Activity: The optimal concentration for FACS analysis, immunoprecipitations and westerns is between 1 and 5 micrograms/ml, but it is recommended that the antibody be titered for optimization before use.

Pre-immune IgG: Pre-immune control IgG purified from pre-immune serum as described above is available.

Expression: mSiglec-F is selectively expressed on murine eosinophils.

References:

Angata T, Hingorani R, Varki NM, et al. Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters. J Biol Chem. 2001;276:45128-45136.

Zhang J, Biedermann B, Nitschke L, et al. The murine inhibitory receptor mSiglec-E is expressed broadly on cells of the innate immune system whereas mSiglec-F is restricted to eosinophils. Eur J Immunol. 2004;34:1175-1184.