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Glycan Array Screening

Human and Murine Lectin Preparation for Analysis

Amount of Lectin Required for Primary Global Screening Protocol:	Participating Investigator (PI) supplies Core H with 1 to 2 mg purified lectin Number of unique ligands: 115 Replicates: 3 Positive Controls: 3 Negative Controls: 3 Volume per well: 50ml Total volume lectin required: 17.25ml Lectin concentration: 30mg/ml Total FITC-labeled Lectin or Igchimera required per primary screening: 518mg
FITC-Labeling of Lectins:	Alexa Fluor 488 Protein Labeling Kit (A-10235) from Molecular Probes Minimum requirement: 1mg/0.5ml Lectin labeled and purified per manufacturer’s protocol
Ig-chimera Analysis Using FITC-Labeled Secondary Antibodies:	PI specifies primary host or Ig used Core H will provide Alexa-labeled secondary antibody
Special Conditions:	Participating Investigators are to contact Rick Alvarez, Director of Core H , prior to sending lectins for analysis [special considerations on a case by case basis]

Glycan Array Screening Available

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Last Updated Tuesday, 26-Aug-2003 16:54:46 EDT . Please contact us with comments/questions.

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