| Family | Zebrafish Gene | Phenotypic Description | Image #1 | Image #2 | Image #3 | Image #4 |
|---|
| hi1 | Cleavage and polyadenylation specificity factor 4 | day 4: small head and eye, a bit of pericardial edema, underdeveloped liver/gut |  | | | |
| hi2 | pescidillo | day 3: small head and eye, round grey yolk; day 5: small head and eye, very small liver and underdeveloped gut, maybe a bit of pericardial edema |  | | | |
| hi4 | dead eye/NIC96-like | day 2: cns necrosis; day 5: very small head/eyes, underdeveloped liver/gut, a bit of pericardial edema |  |  | | |
| hi5 | NAC alpha chain | day 3: beginning necrosis in head, rounder grey yolk, slow circulation; day 4: head an body necrotic, swollen grey yolk; often dead by day 5 |  |  | | |
| hi10 | 60S ribosomal protein L36a | day 1: pinched midbrain/hindbrain boundary, a bit of cns necrosis; day 2: small head and eyes, inflated hindbrain ventricle, thin body, rounder yolk with thin externsion; most dead by day 5 |  |  | | |
| hi14 | eukaryotic translation elongation factor 1 gamma | day 5: swim bladder minus (variable penetrance!), nothing else visibly wrong (NOTE: escapers can be viable, but are much smaller than wild type fish) |  |  | | |
| hi37 | novel, fish specific | days 3-4: slightly smaller eyes, smaller rounder head with thick jaw, grey yolk, some with pc edema (note: 2911B more severe than 37 on day 3, often dead/dying by day 4); day 5: often deteriorating; if not: small head and eyes, underdeveloped liver/gut, often have pericardial edema |  |  | | |
| hi43 | novel, fish specific | day 5: a bit smaller head and eyes, slightly underdeveloped liver/gut |  | | | |
| hi61 | PRDM5/PFM2 | day 5: slightly rounder head, slightly underdeveloped liver/gut |  | | | |
| hi63 | Nop14-like | day 1: pinched midbrain/hindbrain boundary; day 2: small head and eyes, a bit thinner body, rounder yolk with thin externsion; day 5: small head and eyes, a bit thinner body, round grey yolk, underdeveloped liver/gut, pericardial edema |  |  |  | |
| hi96 | sec5 homolog | day 4-5: pericardial edema, also edema around head, eyes and/or yolk; thick looking jaw |  |  | | |
| hi112 | V-ATPase Ac45 | day 2: no/less body pigment; day 5: less body pigment, small head and eyes, underdeveloped liver/gut |  | | | |
| hi138 | heatshock cognate 70 | day 1: pinched midbrain/hindbrain boundary, a bit of cns necrosis; day 2: small head and eyes, inflated hindbrain ventricle, thin body, rounder yolk with thin externsion; day 5: very small head and eyes, thin body with underdeveloped liver/gut |  |  | | |
| hi199 | splicing factor U2AF 35kd | day 1: pinched midbrain/hindbrain boundary, a bit of cns necrosis; day 2: cns necrosis, small head and eyes, inflated hindbrain ventricle, thin body, rounder yolk with thin externsion; dead by day 5 |  | | | |
| hi215 | DEAD/H box 55 RNA helicase | day 1: pinched midbrain/hindbrain boundary, a bit of cns necrosis; day 2: a bit smaller head and eyes, round grey yolk with thin extension; day 5: small head and eyes, round grey yolk, thin wasting body & underdeveloped guts, sometimes a bit of pericarcial edema |  | | | |
| hi229 | smoothened | day 1: U-shaped somites, mildly cyclopic; day 2: smaller eyes, too close together, pc edema denting yolk, which is grey and bloated, body thin and curled ventrally with U-shaped somites, absence of lateral floor plate and secondary motoneurons; usually dead by day 5 (more details in Chen, et al., Development128, 2385-2396 (2001)) |  |  | | |
| hi239 | novel (FLJ14909-like) | day 3: slightly smaller head and eyes; day 5 narrower head, slightly smaller eye, a bit of pc edema, underdeveloped liver/gut |  |  | | |
| hi258 | 60S ribosomal protein L35 | day 1: midbrain/hindbrain boundary a bit thin, hindbrain ventrice inflated, a bit of cns necrosis; day 2: small head and eyes, inflated hindbrain ventricle, thin body, rounder yolk with thin externsion; most dead by day 5 |  |  | | |
| hi272 | nuclear protein NP95 | day 5: a bit smaller head and eyes, smaller liver, slightly underdeveloped gut |  | | | |
| hi286 | MBD2-interacting zinc finger | day 2: cns necrosis, bit smaller head and eyes; day 5: very small head and eyes, underdeveloped liver/guts and pericardial edema |  |  | | |
| hi296a | novel (MGC4607-like) | day 2: enlarged atrium, pericardial edema, denting yolk; day 3: edema/heart defect more apparent, blood pooling in heart cavity; day 5; small head and eyes, thick jaw, pericardial edema (may swell to include yolk area), grey or edema-swollen yolk and underdeveloped liver/gut |  |  |  | |
| hi296b | ski-interacting protein | day 1: cns necrosis, flat head; day 2 very bad necrosis; most dead by day 5 |  |  | | |
| hi297 | novel (FLJ10498-like) | day 3: begin small head and eyes, round grey yolk; day 5: small head and eye, no jaw area, underdeveloped liver/gut, often a bit of pericardial edema |  |  |  | |
| hi299 | novel (nucleostemin-like ) | day 3: small head and eyes, round grey yolk; day 5: small head and eyes, underdeveloped liver/gut, often a bit of pericardial edema |  |  | | |
| hi307 | glucuronosyl-transferase | day 5: slightly smaller and rounder head, looks blockheaded (jaw does not extend anterior to the eyes); Alcian blue does not stain cartilage well, and cartilages are a bit shortened |  |  | | |
| hi318 | ribonucleoside reductase R1 | day 1: small amount of cns necrosis; day 5: narrow/pointy head, a bit smaller eyes, often wavy (S-shaped) body |  | | | |
| hi319 | MCM3 | day 2: a little necrosis in tectum; day 5: a bit smaller head, more so smaller eyes, sometimes slightly underdeveloped liver/gut, sometimes do inflate swim bladder |  | | | |
| hi340a | claudin j | day 2-5: no otoliths |  |  | | |
| hi340b | Exosome complex exonuclease RRP41 | day 2: slightly smaller head and eyes; day 3: smaller head and eyes, rounder yolk; day 5: smaller head and eyes, underdeveloped liver and gut |  |  | | |
| hi343 | tRNA nucleotidyl transferase-like | day 5: a bit smaller/narrower head/eyes, slightly underdev guts |  | | | |
| hi349 | pou2 | day 1: funny midbrain/hindbrain boundary, day 2: short body; day 3-5: short body, head a bit smaller, ears lack otoliths, odd shaped (more details in S. Burgess et al., Development 129: 905-916. (2002)) |  | | | |
| hi383a | TCP-1 gamma | day 3: cns necrosis and small head and eyes, rounder yolk with thin extension; day 5: small head and eyes, round grey yolk, underdeveloped liver/gut |  | | | |
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