Tha Resource Guide

Introduction

1) Structure of DNA and its applications

• Packing into chromosomes
  

• Base pairing
  

• Base pairing (more detail)
  

• Nucleic acid hybridization
  

• Replication
  

• Proteins involved in replication
  

• Gene structure (introns and exons)

2) Transcription and processing

• Central Dogma

• The central dogma of molecular biology

• From DNA to Protein

• Transcription

• Illustration of transcription

• Transcript Modification

• Splicing

• RNA Synthesis and Processing

• Intron/Exon Structures

3) Translation and protein structure

• Translation and the Genetic Code

• Translation

• Protein Synthesis

• Protein Synthesis (detail)

• Coding

• The Genetic Code

• Protein Structure

• Mutations
  

4) Molecular biology tools and techniques:

• Recombinant DNA Technology and Cloning

• Brief Synopsis of Cloning

• Alternate Synopsis of Cloning

• Restriction Endonucleases

• Action of EcoR1

• Blotting (Northern/Southern/Western) Blotting A Primer on Blotting

• Southern Blotting

• PCR

• Sequencing

• Libraries and chips (applications of nucleic acid hybridization)

• Construction of a genomic library

• Microarray Technology

• DNA Chip Fact Sheet

• Practice Problems (with solutions)

5) Mitosis and meiosis

• Mitosis

• Meiosis

• Comparison of Mitosis and Meiosis

• Recombination

• Crossing over during meiosis

• chromosome structure

6) Mendelian genetics

• Resource Text

• Nature of inheritance

• Dominant versus recessive transmission

• X-linked transmission

• Pedigrees

• More Practice Problems (with solutions)

7) Mitochondria and mitochondrial DNA

• Here are two brief exercises that explain mitochondrial DNA and modes of inheritance:

  • Mystery of the Romanovs

  • The Neandertal Mystery

8) Control of gene expression (esp. eukaryotes)

• Regulation of expression Intro

• Regulatory elements (promoters and enhancers)

• Regulatory elements

• Alternate regulatory elements

• Alternate regulatory elements 2

• Analysis of Regulatory Elements

• Transcription factors

• Methods used to study the control of gene expression Gel Shifts & footprinting Reference Materials:

9) Glossaries

• NIH Glossary of Genetic Terms

• Genetics Glossary from Human Genome Project

Of additional interest:

• Forensic Genetics

  • Forensic Genetics The Science of Crime

  • DNA on the Witness Stand

  • Use of DNA in Identification

  • PCR in Forensic Science

  • DNA Fingerprinting-An Interview with Bruce Weir

• Biotechnology and Bioinformatics

  • Applied Biotechnology Areas of Applied Biotechnologyz

  • Biotechnology Links

  • Bioinformatics