February 2002

Molecular Biology

Plasmid Prep: Large Scale Alk Lys

Rubidium Cl Method for Making Competant E coli

Cloning: Tips on cloning/ligating

PCR Screen for Ligation Colonies

DNA Sequencing Notes

Protein Expression in E. coli

Fermenting E. coli

Inclusion Body Prep

Urea HQ pur of IBs

Folding and Purifying DR1-peptide

DR Elisa

DR oligomer T cell staining protocols

Staining with DR1 oligomers, step by step

Staining with DR1 oligomers, detailed

Peptide Binding to DR alleles

EpiPred Prediction Program (Microsoft Excel sheets)

T cell protocols

Thawing and stimulating HA1.7/Cl-1 T cell clones

PBMC prep

T cell Proliferation Assay

CFSE labeling

In vitro Stimulation

In vitro Stim for staining

Anergization of T cells

Calcium Release Assay in Fluorometer

Intracellular Cytokine Assay

S2 cell protocols

S2 cell culture and transfection

Peptide Synthesis

Peptide Synthesis Notes and Directions

Miscellaneous

DR peptide binding competition assay

Labeling SA

OPA assay for free amines

Double western, chemilum and ppting

Acid Strip Protocol

293S cell culture

DR typing by PCR

Various Constructs