The shRNA Validation Project

Included in our database are validated sequences from the shRNA Validation Project, a joint project sponsored by the Integrative Cancer Biology Program (ICBP) and the Cancer Gene Anatomy Project (CGAP). Only those sequences tested as part of the shRNA Validation Project and shown to produce knockdown of mRNA by >70% in two cell lines or greater than >80% in one cell line with four replicates are included in our database.

About the project . . .

"The purpose of this project was to design and test a set of shRNAs for validation as tools to target high priority cancer genes. After validation, the target sequences of the shRNAs were deposited into the NCI's Cancer Genome Anatomy Project (CGAP) open access, public database. All shRNA constructs are commercially available. The validated shRNAs are designed to serve as tools for researchers to target and modulate the expression of well-known cancer-associated genes."

For further information, including validation methods, please visit the CGAP website at: http://cgap.nci.nih.gov/RNAi/shRNAValidation