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GL3 - #1008


Target gene:
P.pyralis (firefly) luciferase gene M15077
Probe type:
siRNA
Sequence:
UCGAAGUACUCAGCGUAAGdTdT
NCBI Database ID#:
8808665
"Sense" sequence:
CUUACGCUGAGUACUUCGAdTdT
 
Comments:  

Publications

 
Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411(6836):494.

Validation Experiments

 
Organism:
Human
Experiment subject:
Cell culture
Cell type:
Kidney Cells
Cell line:
293T
Gene of origin:
Transfected
Treatment prior to RNAi:
N/A
Vector:
N/A
Delivery method:
Lipofectamine 2000
mRNA suppression (%):
Not tested
Method of mRNA detection:
N/A
Protein suppression (%):
90-100
Method of protein suppression:
Luciferase assay
Phenotype observed:
Notes:

Submitters Information

 
Submitted by:
Amanda Garfinkel
Location
KI
Lab
Phillip Sharp
 
 

Last updated January 9th, 2009