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VEGF-A (A3)


Target gene:
VEGF-A NM_001025366
Probe type:
shRNA

Sequence:
CCGGTGGAGTACCCTGATGAGATC
TTCAAGAGA
GATCTCATCAGGGTACTCC
TTTTTTG

NCBI Database ID#:
not submitted
Target sequence:
GATCTCATCAGGGTACTCC
 
Comments:  

Publications

 
Wong SY, Haack H, Crowley D, Barry M, Bronson RT, Hynes RO. 2005. Tumor-secreted vascular endothelial growth factor-C is necessary for prostate cancer lymphangiogenesis, but lymphangiogenesis is unnecessary for lymph node metastasis. Cancer Res. 65:9789

Validation Experiments

 
Organism:
Human
Experiment subject:
cell culture
Cell type:
Prostate adenocarcinoma
Cell line:
PC3-#82
Gene of origin:
Endogenous
Treatment prior to RNAi:
N/A
Vector:
pSIRISP
Delivery method:
Virus infection
mRNA suppression (%):
70-80
Method of mRNA detection:
Real-time PCR
Protein suppression (%):
Not tested
Method of protein suppression:
N/A
Phenotype observed:
Notes:
Knockdown experiments conducted on stably-infected cells that were elected on puromycin (2.5 ug/mL).
PC3-#82 is a lab-generated derivative of the PC-3 cell line.
pSIRISP vector obtained from W.C. Hahn, Dana Farber Cancer Institute.

Submitters Information

 
Submitted by:
Sunny AWong
Location
KI
Lab
Richard Hynes
 
 

Last updated January 9th, 2009