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DGCR8 |
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| Target gene: DGCR8 NM_033324 |
Probe type: shRNA |
| Sequence: GATCCCCCCAATGATGACCA AGATTATTCAAGAGATAATC TTGGTCATCATTGGTTTTTGGAAC |
NCBI Database ID#: not submitted |
| Target sequence: TAATC TTGGTCATCATTGG |
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| Comments: shRNA vector is currently available from Addgene. |
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Publications |
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| Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. 2007. Impaired microRNA processing enhances cellular transformation and tumorigenesis. Nat Genet. 39:673 | |
Validation Experiments |
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| Organism: Mouse |
Experiment subject: Cell culture |
| Cell type: Lung adenocarcinoma |
Cell line: LKR13 |
| Gene of origin: Endogenous |
Treatment prior to RNAi: |
| Vector: pSuper.retro.puro |
Delivery method: Virus infection |
| mRNA suppression (%): 80% |
Method of mRNA detection: real-time PCR |
| Protein suppression (%): N/A |
Method of protein suppression: N/A |
| Phenotype observed: | |
| Notes: |
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Submitters Information |
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| Submitted by: Madhu Kumar |
Location KI |
| Lab Tyler Jacks |
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Last updated January 9th, 2009