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Dicer1


Target gene:
Dicer1 NM_026799
Probe type:
shRNA
Sequence:
GATCCCCGCATGGTGGTGTC GATATTTTCAAGAGAAATAT CGACACCACCATGCTTTTTGGAAC
NCBI Database ID#:
not submitted
Target sequence:
AATATCGACACCACCATGC
 
Comments:
shRNA vector is currently available from Addgene.

Publications

 
Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. 2007. Impaired microRNA processing enhances cellular transformation and tumorigenesis. Nat Genet. 39:673

Validation Experiments

 
Organism:
Mouse
Experiment subject:
Cell culture
Cell type:
Lung adenocarcinoma
Cell line:
LKR13
Gene of origin:
Endogenous
Treatment prior to RNAi:
Vector:
pSuper.retro.puro
Delivery method:
Virus infection
mRNA suppression (%):
80%
Method of mRNA detection:
real-time PCR
Protein suppression (%):
N/A
Method of protein suppression:
N/A
Phenotype observed:
Notes:

Submitters Information

 
Submitted by:
Madhu Kumar
Location
KI
Lab
Tyler Jacks
 
 

Last updated January 9th, 2009