Small Scale (Multiple) 1-10umol
General Information
We use the Intavis Model MultiPep multiple peptide synthesizer to make up to 192 peptides simultaneously. The synthesis scale is 1-10umol, with 5umol being the most common. This service is ideal for peptide library and screening experiments. Applications include protein binding interactions, epitope mapping, enzyme inhibition and immunodiagnostics. Starting with tentagel resin, the peptide is built "backwards," from the C-terminus to the N-terminus. After synthesis, the peptide is cleaved from the resin, yielding approximately 2.5µmoles of the crude form of the peptide. Generally, this type of synthesis yields crude peptides with 80-85% purity, but HPLC purification can be done if higher purity is desired. Average peptide lengths are 10-20 amino acids. In a typical run, 0-5% of the peptides fail to synthesize properly due to difficulties in coupling caused by the specific amino acid sequence of the peptide. No feedback to adjust chemistry during run but even 50mers may be achievable.
The Final Product
The peptide, in both crude and purified forms, is weighed and given out as dry powder . Mass spectrometry data is provided as well HPLC data.
1. Assume that 50% of the synthesis scale is present for each peptide.
2. Assume that about 75% by weight is peptide, with the remainder being H20 and TFA salt.
