You have made your way from worm to man, and much within you is still worm.
How do genes control animal development and behavior? To answer this question, weuse the experimentally tractable nematode Caenorhabditis elegans to identify and analyze molecular and cellular pathways involved in these important areas of biology, with the goal of discovering fundamental biological mechanisms and revealing insights into human diseases.
Naturally-occurring or “programmed” cell deaths play majorroles in animal development and homeostasis, and abnormalities in programmed cell deaths are causally involved in numerous human diseases, including cancer and certain neurodegenerative disorders. Diverse developmental events involve programmed cell deaths. The loss of the tadpole tail during metamorphosis into a frog, the sculpting of human fingers and toes in utero, the seasonal degeneration of the brain region that drives birdsong, and the elimination of self-reactive cells in the immune system all provide examples of programmed cell death. Cells that die by programmed cell death undergo a process known as apoptosis, defined by morphological and ultrastructural characteristics. Abnormalities in programmed cell death are causally involved in numerous human diseases, including certain neurodegenerative disorders and cancer. We discovered that the 131 programmed cell deaths that occur during C. elegans development are controlled by a specific set of killer and protector genes, and we defined the canonical molecular genetic pathway for programmed cell death. This pathway of cellular suicide subsequently proved to be conserved in other animals, including humans, and the human homologs of genes we discovered are now therapeutic targets for human diseases. One of the C. elegans killer genes, ced-3, encodes the founding member of a family of proteases known as caspases, key drivers of apoptotic cell death. We recently discovered that in C. elegans some cells die in the absence of caspases. We currently are exploring a number of major problems in the field of cell death:
Animal development begins with the fertilized egg andproceeds through many cell divisions to generate a great variety of different cell types. How cell diversity is generated during development is a fundamental issue in biology. C. elegans has only 959 somatic cells and a known cell lineage, facilitating analyses of development at the single-cell level. We have identified mechanisms that make daughter cells different from mother cells, that makesister cells different from each other or that break left-right developmental symmetry. Our studies of the induction of vulval development helped define the Ras signal transduction pathway, key in cancer biology. We defined the heterochronic pathway, which controls developmental timing and led to the discovery of microRNAs. We currently are analyzing the following issues involving cell lineage and cell fate:
To explore the molecular, cellular and circuit bases of nervous systemfunction, we are analyzing the coordinately controlled C. elegans behaviors of feeding, egg laying and locomotion. From our studies of behavior we have identified founding members of the RGS protein family, which regulates signaling from G-protein coupled receptors, and the EGLN family of dioxygenases, which control the HIF-VHL pathway that is vital in cellular and organismic O2 sensing and is a therapeutic target in cancer. We also identified a novel class of neurotransmitter receptors, chloride channels gated by biogenic amines, and a new neurotransmitter, tyramine. Our current studies include the following problems:
We have long studied the humanneurodegenerative disease ALS. In collaborative studies, we have identified and characterized many ALS genes, including the first known ALS gene, SOD-1. We are now analyzing: