We are currently distributing many of our reagents through the non-profit company Addgene. These reagents can be ordered directly from Addgene by using the following link: Sharp Lab Plasmids

For enquiries into reagents not currently available through Addgene, please email mwernett@mit.edu.

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Sharp Lab Reagents Available through Addgene:

Reference: siRNAs can function as miRNAs. Doench JG et al. (Genes Dev. 2003 Feb 15. 17(4):438-42. Pubmed)

pRL-TK CXCR4 2x
pRL-TK CXCR4 4x
pRL-TK CXCR4 6x
pRL-TK CXCR4 1P
pGL3 CXCR4 1P
pGL3 CXCR4 2P
pGL3 CXCR4 3P

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Reference: Specificity of microRNA target selection in translational repression. Doench JG et al. (Genes Dev. 2004 Mar 1. 18(5):504-11. Pubmed)

pRL-TK 4x wt
pRL-TK 4x mut A
pRL-TK 4x mut B
pRL-TK 4x mut C
pRL-TK 4x mut D
pRL-TK 4x mut E
pRL-TK 4x mut F
pRL-TK 4x mut G
pRL-TK 4x mut H
pRL-TK 4x mut I
pRL-TK 4x mut GFP
pRL-TK let7 A
pRL-TK let7 B
pRL-TK let7 C
pRL-TK let7 D
pRL-TK let7 E
pRL-TK let7 F
pRL-TK let7 G
pRL-TK let7 H
pRL-TK let7 I

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Reference: Regulation of CD44 Alternative Splicing by SRm160 and Its Potential Role in Tumor Cell Invasion. Cheng C et al. (Mol Cell Biol. 2006 Jan . 26(1):362-70. Pubmed)

Flag-SRm160

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Reference: Short RNAs Repress Translation after Initiation in Mammalian Cells. Petersen CP et al. (Mol Cell. 2006 Feb 17. 21(4):533-42. Pubmed)

pFR_CrPV_xb
pFR_HCV_xb
pReadthroughUAG
pReadthroughUAA
pReadthroughUGA