Sharp Lab
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Many reagents appearing in Sharp Lab publications have been submitted to the non-profit distribution company Addgene either by the Sharp Lab or by collaborating labs. These reagents can be ordered directly from Addgene by going to http://www.addgene.org/.

For enquiries into reagents not currently available through Addgene, please email mwernett@mit.edu.


Reagents Available through Addgene:

Reference: Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Garcia-Blanco MA, Jamison SF, Sharp PA (Genes Dev. 1989 Dec . 3(12A):1874-86. PubMed)

pGEX-2TK-PTB


Reference: siRNAs can function as miRNAs. Doench JG et al. (Genes Dev. 2003 Feb 15. 17(4):438-42. Pubmed)

pRL-TK CXCR4 2x
pRL-TK CXCR4 4x
pRL-TK CXCR4 6x
pRL-TK CXCR4 1P
pGL3 CXCR4 1P
pGL3 CXCR4 2P
pGL3 CXCR4 3P


Reference: Specificity of microRNA target selection in translational repression. Doench JG et al. (Genes Dev. 2004 Mar 1. 18(5):504-11. Pubmed)

pRL-TK 4x wt
pRL-TK 4x mut A
pRL-TK 4x mut B
pRL-TK 4x mut C
pRL-TK 4x mut D
pRL-TK 4x mut E
pRL-TK 4x mut F
pRL-TK 4x mut G
pRL-TK 4x mut H
pRL-TK 4x mut I
pRL-TK 4x mut GFP
pRL-TK let7 A
pRL-TK let7 B
pRL-TK let7 C
pRL-TK let7 D
pRL-TK let7 E
pRL-TK let7 F
pRL-TK let7 G
pRL-TK let7 H
pRL-TK let7 I


Reference: Regulation of CD44 Alternative Splicing by SRm160 and Its Potential Role in Tumor Cell Invasion. Cheng C et al. (Mol Cell Biol. 2006 Jan . 26(1):362-70. Pubmed)

Flag-SRm160


Reference: Short RNAs Repress Translation after Initiation in Mammalian Cells. Petersen CP et al. (Mol Cell. 2006 Feb 17. 21(4):533-42. Pubmed)

pFR_CrPV_xb
pFR_HCV_xb
pReadthroughUAG
pReadthroughUAA
pReadthroughUGA


Reference: Quantitative Analysis of Argonaute Protein Reveals MicroRNA-Dependent Localization to Stress Granules. Leung AK, Calabrese JM, Sharp PA (Proc Natl Acad Sci U S A. 2006 Nov 28. 103(48):18125-30. PubMed)

EGFP-hAgo2


Reference: MicroRNA Sponges: Competitive Inhibitors of Small RNAs in Mammalian Cells. Ebert MS, Neilson JR, Sharp PA (Nat Methods. 2007 Sep . 4(9):721-6. Pubmed)

CMV-d2eGFP-cxcr4
CMV-d2eGFP-16pf
CMV-d2eGFP-20
CMV-d2eGFP-20pf
CMV-d2eGFP-21
U6+27-16
U6+27-16pf
U6+27-20
U6+27-20pf
CMV-d2eGFP-16
U6+27-cxcr4
CMV-d2eGFP-empty*

* The paper used the cxcr4 vector as a control. The empty vector can be used as a control for the CMV-d2eGFP plasmids in the paper, but the multiple cloning site has been destroyed and this construct should not be used for further cloning.


Reference: Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar MS, Erkeland SJ, Pester RE, Chen CY, Ebert MS, Sharp PA, Jacks T.(Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3903-8. Pubmed)

Puro.Cre empty vector
MSCV puro let-7 sponge
Puro.let-7gsm.Cre
Puro.let-7g.Cre
pBabe zeo HMGA2


Reference: Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland SJ, Newman J, Bronson RT, Crowley D, Stone JR, Jaenisch R, Sharp PA, Jacks T (Cell. 2008 Mar 7. 132(5):875-86. Pubmed)

pBS-miR106a-363
pKO-II-miR-17~92
pKS-DTA miR-106b~25


Reference: MicroRNAs can generate thresholds in target gene expression. Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA, van Oudenaarden A (Nat Genet. 2011 Aug 21;43(9):854-9. Pubmed)

pTRETightBI-RY-0
pTRETightBI-RY-1
pTRETightBI-RY-4
pTRETightBI-RY-7
pTRETightBI-RY-1pf
pTRETightBI-RY-HMGA2wt
pTRETightBI-RY-HMGA2mutant
pTRETightBI-RY-SLC6A1


Reference:In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. Gurtan AM, Lu V, Bhutkar A, Sharp PA (RNA. 2012 Jun;18(6):1116-22. Pubmed)

pCAGGS-mCherry
pCAGGS-Flag-hsDicer
pCAGGS-Flag-hsDicer (D1320A/D1709A)
pCAGGS-Flag-hsDicer (D1320A)
pCAGGS-Flag-hsDicer (E1564A)
pCAGGS-Flag-hsDicer (D1709A)
pCAGGS-Flag-hsDicer (K70A)
pCAGGS-Flag-hsDicer (Y971A/Y972A)


Reference: A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails. Wilusz JE, JnBaptiste CK, Lu LY, Kuhn CD, Joshua-Tor L, Sharp PA. (Genes Dev. 2012 Nov 1;26(21):2392-407. Pubmed)

pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense
pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense
pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense
pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense
pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

MIT Koch Institute for Integrative Cancer Research at MIT