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siRL3 - #1001
Target gene:
Renilla luciferase AAB82579		 Probe type:
siRNA
Anti-sense sequence:
UAUUAGGAAACUUCUUGGCUU		 NCBI Database ID#:
8694723
Sense sequence:
GCCAAGAAGUUUCCUAAUAUU 		 
Comments:  

Publications

  
Calabrese JM, Sharp PA. 2006, Characterization of the short RNAs bound by the P19 suppressor of RNA silencing in mouse embryonic stem cells. RNA v.12(12):2092.

Validation Experiments

  
Organism:
Human		 Experiment subject:
cell culture
Cell type:
kidney		 Cell line:
293T
Gene of origin:
Transfected		 Treatment prior to RNAi:
n/a
Vector:
n/a		 Delivery method:
lipofectamine 2000
mRNA suppression (%):
Not tested		 Method of mRNA detection:
Not tested
Protein suppression (%):
80-90%		 Method of protein suppression:
luciferase assay
Phenotype observed:
Notes:
Protein expression assayed 24 hours post-transfection.

Submitters Information

  
Submitted by:
Mauro Calabrese		 Location
KI
Lab
Phillip Sharp		  
 
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Last updated January 9th, 2009