shRNA Submission
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Before submitting your shRNA data, please review our submission guidelines.
*Fields not required for acceptance are marked (optional)*
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| Personal Information (more info...) |
| Name: |
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| Email: |
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| Title: |
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| Principle investigator: |
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| Department or center: |
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| NCBI Probe Database (more info...) |
| If you have previously submitted your siRNA sequence to the NCBI Probes Database, we ask you to provide the NCBI Probe reference number to allow us to link to your submitted data. If you have not submitted your data through NCBI, we will submit it for you based on the information provided below. |
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Probe ID #: |
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| Reference (more info...) |
| Please provide the Pubmed ID# of your publications that first describe the siRNA sequence you are submitting. If the article is currently in press, we request that you provide us with the article title, authors, and journal so that we can provide links upon publication. |
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Pubmed ID#: |
(search for PMID. . .) |
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Title: |
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Authors: |
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Journal: |
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Unpublished |
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| Probes (more info...) |
| Please provide information on your siRNA below. If you have additional information regarding the siRNA, please enter that information in the "comments" field. |
| Probe name: |
[?]
(name to describe shRNA; can use gene name for this) |
| Probe type: |
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Please provide the mRNA RefSeq accession number (NM_ or XM_ accession number) for your target gene. You may follow the link out to the NCBI site to search for the gene. Please see our "Faqs" for additional tips on obtaining the proper accesion number. |
| Target mRNA accession #: |
(search for accession number . . .) |
| Target mRNA name: |
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Full shRNA sequence (5'-3'): |
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| Target mRNA sequence: |
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Please provide the nucleotide positions of both the "sense" and "anti-sense" sequence within your shRNA.
Note that the first nucleotide of the shRNA is considered to be position "0", not "1".
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Sense sequence position: |
ex. 7:25 |
| Anti-sense sequence position: |
ex. 36:54 |
Comments (optional):
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| Results (more info) |
The MIT/ICBP siRNA Database will only accept shRNAs that have been shown to work experimentally. |
| Experiment name: |
(ex. Rb knockdown in HeLa Cells, 3/28/07) |
| Organism: |
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| Experiment subject: |
Cell culture
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| Cell type: |
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| Cell line: |
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Gene of origin : |
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| Treatment prior to RNAi (optional): |
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| Vector (optional): |
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| Dose (optional): |
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| Delivery method: |
If "other", please specify
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shRNA validation can be in the form of mRNA suppression and/or protein suppression levels.
You must supply one of these for your submission to be accepted.
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| mRNA suppression: |
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| Method of mRNA detection: |
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| Protein suppression: |
(% reduction) |
| Method of protein detection: |
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Phenotype observed (optional):
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Notes (optional) :
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