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Xrn1-Delta - #1007 |
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| Target gene: Xrn1 NM_019001 |
Probe type: siRNA |
| Sequence: UUAGUAGGUACUUGAGUAGdTdT |
NCBI Database ID#: 8808664 |
| "Sense" sequence: CUACUCAAGUACCUACUAAdTdT |
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| Comments: | |
Publications |
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| Alemán LM, Doench J, Sharp PA, 2007. Comparison of siRNA-induced off-target RNA and protein effects. RNA 13(3):385 | |
Validation Experiments |
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| Organism: Human |
Experiment subject: Cell culture |
| Cell type: Cervical Carcinoma |
Cell line: Hela |
| Gene of origin: Endogenous |
Treatment prior to RNAi: N/A |
| Vector: N/A |
Delivery method: Oligofectamine |
| mRNA suppression (%): 90-100 |
Method of mRNA detection: Real-time PCR |
| Protein suppression (%): Not tested |
Method of protein suppression: N/A |
| Phenotype observed: | |
| Notes: Results seen with 100nM mixture of XRN-1 Beta + XRN-1 Delta, equamolar concentrations of each. |
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Submitters Information |
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| Submitted by: Amanda Garfinkel |
Location KI |
| Lab Phillip Sharp |
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Last updated January 9th, 2009