MIT siRNA Website

return to gene table

Xrn1-Delta - #1007


Target gene:
Xrn1 NM_019001
Probe type:
siRNA
Sequence:
UUAGUAGGUACUUGAGUAGdTdT
NCBI Database ID#:
8808664
"Sense" sequence:
CUACUCAAGUACCUACUAAdTdT
 
Comments:  

Publications

 
Alemán LM, Doench J, Sharp PA, 2007. Comparison of siRNA-induced off-target RNA and protein effects. RNA 13(3):385

Validation Experiments

 
Organism:
Human
Experiment subject:
Cell culture
Cell type:
Cervical Carcinoma
Cell line:
Hela
Gene of origin:
Endogenous
Treatment prior to RNAi:
N/A
Vector:
N/A
Delivery method:
Oligofectamine
mRNA suppression (%):
90-100
Method of mRNA detection:
Real-time PCR
Protein suppression (%):
Not tested
Method of protein suppression:
N/A
Phenotype observed:
Notes:
Results seen with 100nM mixture of XRN-1 Beta + XRN-1 Delta, equamolar concentrations of each.

Submitters Information

 
Submitted by:
Amanda Garfinkel
Location
KI
Lab
Phillip Sharp
 
 

Last updated January 9th, 2009