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GL3 - #1008 |
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| Target gene: P.pyralis (firefly) luciferase gene M15077 |
Probe type: siRNA |
| Sequence: UCGAAGUACUCAGCGUAAGdTdT |
NCBI Database ID#: 8808665 |
| "Sense" sequence: CUUACGCUGAGUACUUCGAdTdT |
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| Comments: | |
Publications |
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| Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411(6836):494. | |
Validation Experiments |
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| Organism: Human |
Experiment subject: Cell culture |
| Cell type: Kidney Cells |
Cell line: 293T |
| Gene of origin: Transfected |
Treatment prior to RNAi: N/A |
| Vector: N/A |
Delivery method: Lipofectamine 2000 |
| mRNA suppression (%): Not tested |
Method of mRNA detection: N/A |
| Protein suppression (%): 90-100 |
Method of protein suppression: Luciferase assay |
| Phenotype observed: | |
| Notes: |
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Submitters Information |
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| Submitted by: Amanda Garfinkel |
Location KI |
| Lab Phillip Sharp |
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Last updated January 9th, 2009