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VEGF-A (A3)
Target gene:
VEGF-A NM_001025366 		Probe type:
shRNA

Sequence:
CCGGTGGAGTACCCTGATGAGATC
TTCAAGAGAGATCTCATCAGGGTACTCC
TTTTTTG

 NCBI Database ID#:
not submitted
Target sequence:
GATCTCATCAGGGTACTCC		  
Comments:  

Publications

  
Wong SY, Haack H, Crowley D, Barry M, Bronson RT, Hynes RO. 2005. Tumor-secreted vascular endothelial growth factor-C is necessary for prostate cancer lymphangiogenesis, but lymphangiogenesis is unnecessary for lymph node metastasis. Cancer Res. 65:9789

Validation Experiments

  
Organism:
Human		 Experiment subject:
cell culture
Cell type:
Prostate adenocarcinoma		 Cell line:
PC3-#82
Gene of origin:
Endogenous		 Treatment prior to RNAi:
N/A
Vector:
pSIRISP		 Delivery method:
Virus infection
mRNA suppression (%):
70-80		 Method of mRNA detection:
Real-time PCR
Protein suppression (%):
Not tested		 Method of protein suppression:
N/A
Phenotype observed:
Notes:
Knockdown experiments conducted on stably-infected cells that were elected on puromycin (2.5 ug/mL).
PC3-#82 is a lab-generated derivative of the PC-3 cell line.
pSIRISP vector obtained from W.C. Hahn, Dana Farber Cancer Institute.

Submitters Information

  
Submitted by:
Sunny AWong		 Location
KI
Lab
Richard Hynes		  
 

Last updated January 9th, 2009