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MET


Target gene:
MET (NM_000245)
Probe type:
siRNA
Sense sequence:
CGAGGGAATCATCATGAAATT
NCBI Database ID#:
8810143

Anti-sense sequence:
TTTCATGATGATTCCCTCGGT

 
Comments:  
SNP in nucleotide 17 (T/C)

Publications

 
Martin SE, Jones TL, Thomas CL, Lorenzi PL, Nguyen DA, Runfola T, Gunsior M, Weinstein JN, Goldsmith PK, Lader E, Huppi K, Caplen NJ. Multiplexing siRNAs to compress RNAi-based screen size in human cells. 2007. Nucleic Acids Res. 35:e57

Validation Experiments

 
Organism:
Human
Experiment subject:
Cell culture
Cell type:
Breast cancer
Cell line:
MDA-MB-231
Gene of origin:
Endogenous
Treatment prior to RNAi:
N/A
Vector:
N/A
Delivery method:
siLentfect
mRNA suppression (%):
78%
Method of mRNA detection:
Branched-DNA assay
Protein suppression (%):
93%
Method of protein suppression:
Multi-array ECL
Phenotype observed:
Notes:

Submitters Information

 
Submitted by:
Natasha Caplen
Location
National Cancer Institute
   
 


Last updated January 9th, 2009