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EGFR
(epidermal growth factor receptor)

Target gene:
EGFR (NM_005228)		 Probe type:
siRNA
Sense sequence:
GGATAGTATGAGCCCTAAATT		 NCBI Database ID#:
8810069

Anti-sense sequence:
TTTAGGGCTCATACTATCCTC

 
Comments:
siRNA is against exon 30. Only EGFR variants expressing exon 30 are expected to be targeted.

Publications

  
Martin SE, Jones TL, Thomas CL, Lorenzi PL, Nguyen DA, Runfola T, Gunsior M, Weinstein JN, Goldsmith PK, Lader E, Huppi K, Caplen NJ. Multiplexing siRNAs to compress RNAi-based screen size in human cells. 2007. Nucleic Acids Res. 35:e57

Validation Experiments

  
Organism:
Human		 Experiment subject:
Cell culture
Cell type:
Breast cancer		 Cell line:
MDA-MB-231
Gene of origin:
Endogenous		 Treatment prior to RNAi:
N/A
Vector:
N/A 		Delivery method:
siLentfect
mRNA suppression (%):
75%		 Method of mRNA detection:
Branched-DNA assay
Protein suppression (%):
75%		 Method of protein suppression:
Multi-array ECL
Phenotype observed:
Notes:

Submitters Information

  
Submitted by:
Natasha Caplen		 Location
National Cancer Institute
   
 

Last updated January 9th, 2009