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MAP2K1
(mitogen-activated protein kinase kinase 1)

Target gene:
MAP2K1 (NM_002755)		 Probe type:
siRNA
Sense sequence:
GGATCAAGTCCTGAAGAAATT		 NCBI Database ID#:
8810135

Anti-sense sequence:
TTTCTTCAGGACTTGATCCAG

 
Comments:  
 

Publications

  
Martin SE, Jones TL, Thomas CL, Lorenzi PL, Nguyen DA, Runfola T, Gunsior M, Weinstein JN, Goldsmith PK, Lader E, Huppi K, Caplen NJ. Multiplexing siRNAs to compress RNAi-based screen size in human cells. 2007. Nucleic Acids Res. 35:e57

Validation Experiments

  
Organism:
Human		 Experiment subject:
Cell culture
Cell type:
Colon cancer		 Cell line:
HCT-116
Gene of origin:
Endogenous		 Treatment prior to RNAi:
N/A
Vector:
N/A 		Delivery method:
siLentfect
mRNA suppression (%):
73%		 Method of mRNA detection:
Branched-DNA assay
Protein suppression (%):
66%		 Method of protein suppression:
Western blot
Phenotype observed:
Notes:

Submitters Information

  
Submitted by:
Natasha Caplen		 Location
National Cancer Institute
   
 

Last updated January 9th, 2009