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CDKN2B
(Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4))


Target gene:
CDKN2B (NM_004936)
Probe type:
shRNA
Sequence:
TGCTGTTGACAGTGAGCG
AGGGAATAACCTTCCATACATT
TAGTGAAGCCACAGATGTA
AATGTATGGAAGGTTATTCCCG
TGCCTACTGCCTCGGA
NCBI Database ID#:
Not available
Target sequence:
GGAATAACCTTCCATACAT
 
Comments:  
This sequence is part of the shRNA Validation Project.
This shRNA is available for purchase from Open Biosystems
(Oligo ID =V2LHS_257806)
 

Publications

 
This dataset is unpublished. Further information on the validation experiments can be found at the shRNA Validation Project website.

Validation Experiments

 
Organism:
Human
Experiment subject:
Cell culture
Cell type:
Ovarian cancer / Breast cancer
Cell line:
OVCAR-8 / MCF-7
Gene of origin:
Endogenous
Treatment prior to RNAi:
N/A
Vector:
pGIPZ lentiviral expression vector
Delivery method:
Viral infection

mRNA suppression (%):
82% in OVCAR-8 cells
74% in MCF-7 cells

Method of mRNA detection:
Quantitive real-time PCR
Protein suppression (%):
Not tested
Method of protein suppression:
N/A
Phenotype observed:
Notes:
For further information on this and other shRNA Validation Project entries, visit http://cgap.nci.nih.gov/RNAi/ICBP.

Submitters Information

 
Submitted by:
Mary Lindstrom, based on information provided by the shRNA Validation Project.
Location
NCI
   
 


Last updated January 9th, 2009