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RAF1
(V-raf-1 murine leukemia viral oncogene homolog 1)
Target gene:
RAF1 (NM_002880)		 Probe type:
shRNA
Sequence:
TGCTGTTGACAGTGAGCG
CCGGAGATGTTGCAGTAAAGAT
TAGTGAAGCCACAGATGTA
ATCTTTACTGCAACATCTCCGT
TGCCTACTGCCTCGGA		 NCBI Database ID#:
Not available
Target sequence:
GGAGATGTTGCAGTAAAGA		  
Comments:  
This sequence is part of the shRNA Validation Project.
This shRNA is available for purchase from Open Biosystems
(Oligo ID =V2LHS_171213)
 

Publications

  
This dataset is unpublished. Further information on the validation experiments can be found at the shRNA Validation Project website.

Validation Experiments

  
Organism:
Human		 Experiment subject:
Cell culture
Cell type:
Ovarian cancer / Breast cancer		 Cell line:
OVCAR-8 / MCF-7
Gene of origin:
Endogenous		 Treatment prior to RNAi:
N/A
Vector:
pGIPZ lentiviral expression vector 		Delivery method:
Viral infection

mRNA suppression (%):
91% in OVCAR-8 cells
72% in MCF-7 cells

 Method of mRNA detection:
Quantitive real-time PCR
Protein suppression (%):
Not tested		 Method of protein suppression:
N/A
Phenotype observed:
Notes:
For further information on this and other shRNA Validation Project entries, visit http://cgap.nci.nih.gov/RNAi/ICBP.

Submitters Information

  
Submitted by:
Mary Lindstrom, based on information provided by the shRNA Validation Project. 		Location
NCI
   
 

Last updated January 9th, 2009