Biopolymers Fees
Service |
Description |
KI |
MIT (non-KI) |
|---|---|---|---|
| Peptide Synthesis - 0.1mmol scale | Set-up (includes cleavage, analytical HPLC and MS), per peptide |
$50 | $75 |
| Synthesis, per residue | $20 | $30 | |
| Peptide Synthesis - 0.3mmol scale | Set-up (includes cleavage, analytical HPLC and MS,) per peptide |
$100 | $150 |
| Synthesis, per residue | $28 | $42 | |
| Peptide Synthesis - 0.5mmol scale | Set-up (includes cleavage, analytical HPLC and MS), per peptide |
$150 | $225 |
| Synthesis, per residue | $34 | $50 | |
| N-terminal Biotin | same as per residue synthesis | ||
| Modifications | (5mg/20mg) |
||
Fluorescein (5,6 FAM) |
$73/75 | $109.50/112.50 | |
TAMRA |
$76/86 | $114/129 | |
FMOC Lys (DDE) |
$74/81 | $111/121.50 | |
P-Tyr |
$75/86 | $112.50/129 | |
| Non standard modifications | Inquire | Inquire | |
| HPLC Purification ~100mg crude (peak pooling, analytical HPLC, MS) |
$200 | $300 | |
| Peptide Synthesis-Small Scale (1-10 µmole) includes cleavage, analytical HPLC and MS |
per residue | $2.70 | $4.05 |
| Modifications | |||
N-terminal Biotin |
$2.70 | $4.05 | |
Fluorescein (5,6 FAM) |
$36 | $54 | |
TAMRA |
$37 | $55.50 | |
FMOC Lys (DDE) |
$54 | $81 | |
P-Tyr |
$66 | $99 | |
| Non-standard modifications | Inquire | Inquire | |
| HPLC Purification, per peptide (peak pooling, analytical HLPC, MS) |
$100 | $150 | |
| Desalting, per peptide | $20 | $30 | |
| Cellulose Peptide Array Spot Synthesis | 1 membrane (600 peptides) | $600 | $900 |
| Mass Spec-MALDI-TOF | Analysis, per sample | $22 | $33 |
| Desalting, per sample | $11 | $16.50 | |
| HPLC | Analytical Run | $5 | $7.50 |
| Dye-Labeled Oligonucleotide purification (up to 10mg) | $20 | $30 | |
| Purification with out MALDI analysis (up to 10mg) | $50 | $75 | |
| Small Scale Purification with MALDI analysis (up to 10mg) | $100 | $150 | |
| Large Scale Purification with MALDI analysis(up to 100mg) | $200 | $300 | |
| User Operated Run, analytical scale (0.2ml/min) | $3 | $4.50 | |
| User Operated Run, purification scale (1ml/min) | $4 | $6 | |
| Training | no cost | Time permitting | |
Note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Services are billed at the rate listed on the date of service delivery.
The above rates are effective as of January 1, 2014.
Proteomics Fees
Service |
Description |
KI |
MIT (non-KI) |
|---|---|---|---|
| Simple sample preparation | Sample Digestion, in-gel or in-solution with standard enzyme* | First sample: $100 Second sample: $50 Third to sixth sample: $25 each (applies to samples submitted together) | First sample: $150 Second sample: $75 Third to sixth sample: $37.50 each (applies to samples submitted together) |
| Complex sample preparation | Sample digestion with standard enzyme*, extensive sample cleanup** required (e.g. fractionation, desalting) | First sample: $150 Second sample: $75 Third to sixth sample: $40 each (applies to samples submitted together) | First sample: $225 Second sample: $112.50 Third to sixth sample: $60 each (applies to samples submitted together) |
| LC-MS analysis | High-performance nanoflow HPLC fractionation of peptides with on-line LC-MS analysis | $75 per sample | $112.50 per sample |
| Basic data analysis | Protein identification from database search | Included with LC-MS analysis | Included with LC-MS analysis |
| Medium complexity data analysis | Protein characterization requiring non-automated interpretation of MS and MS/MS data | $30 per sample | $45 per sample |
| Medium complexity data analysis | Protein quantitation (label-free or using isotopic or isobaric labels), GeLC analyses | $55 per hour (with advance estimate) | $82.50 per hour (with advance estimate) |
| MW determination by electrospray ionization mass spectrometry | Intact protein molecular weight measurement – includes on-line sample desalting and deconvolution of electrospray data | $75 per sample | $112.50 per sample |
* We have availabe trypsin, chymotrypsin or endoproteinase AspN for protein digestions; other enzymes may require an additional charge, which will be quoted in advance.
** We use ZipTips® and Centricon® centrifugal filter devices for sample desalting; other materials/media may require an additional charge, which will be quoted in advance.
Note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Services are billed at the rate listed on the date of service delivery.
