Biopolymers & Proteomics Laboratory

The David H.Koch Institute for Integrative Cancer Research at MIT

Bldg 76 Room 181

Telephone: 617-253-7038

Biopolymers Fees

Service
Description
KI
MIT
(non-KI)
Sanger DNA Sequencing Individual samples, per template $5.25 $6.00
Difficult Template, per template
(full scale reaction, blending kits)
$9.00 $10.00
96well plate, per template
(≥75samples in plate)
$4.25 $5.00
Peptide Synthesis - 0.1mmol scale Set-up (includes cleavage, analytical HPLC and MS), per peptide
$50 $75
Synthesis, per residue $20 $30
Peptide Synthesis - 0.3mmol scale Set-up (includes cleavage, analytical HPLC and MS,) per peptide
$100 $150
Synthesis, per residue $28 $42
Peptide Synthesis - 0.5mmol scale Set-up (includes cleavage, analytical HPLC and MS), per peptide
$150 $225
Synthesis, per residue $34 $50
N-terminal Biotin same as per residue synthesis
Modifications
(5mg/20mg)
Fluorescein (5,6 FAM)
$73/75 $109.50/112.50
TAMRA
$76/86 $114/129
FMOC Lys (DDE)
$74/81 $111/121.50
P-Tyr
$75/86 $112.50/129
Non standard modifications Inquire Inquire
HPLC Purification ~100mg crude
(peak pooling, analytical HPLC, MS)
$200 $300
Peptide Synthesis-Small Scale
(1-10 µmole) includes cleavage, analytical HPLC and MS
per residue $2.70 $4.05
Modifications
N-terminal Biotin
$2.70 $4.05
Fluorescein (5,6 FAM)
$36 $54
TAMRA
$37 $55.50
FMOC Lys (DDE)
$54 $81
P-Tyr
$66 $99
Non-standard modifications Inquire Inquire
HPLC Purification, per peptide
(peak pooling, analytical HLPC, MS)

$100 $150
Desalting, per peptide $20 $30
Cellulose Peptide Array Spot Synthesis 1 membrane (600 peptides) $600 $900
Mass Spec-MALDI-TOF Analysis, per sample $22 $33
Desalting, per sample $11 $16.50
HPLC Analytical Run $5 $7.50
Dye-Labeled Oligonucleotide purification (up to 10mg) $20 $30
Purification with out MALDI analysis (up to 10mg) $50 $75
Small Scale Purification with MALDI analysis (up to 10mg) $100 $150
Large Scale Purification with MALDI analysis(up to 100mg) $200 $300
User Operated Run, analytical scale (0.2ml/min) $3 $4.50
User Operated Run, purification scale (1ml/min) $4 $6
Training no cost Time permitting

Note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Services are billed at the rate listed on the date of service delivery.

The above rates are effective as of January 1, 2014.

Proteomics Fees

Service
Description
KI
MIT (non-KI)
Simple sample preparation Sample Digestion, in-gel or in-solution with standard enzyme* First sample: $100 Second sample: $50 Third to sixth sample: $25 each (applies to samples submitted together) First sample: $150 Second sample: $75
Third to sixth sample: $37.50 each (applies to samples submitted together)
Complex sample preparation Sample digestion with standard enzyme*, extensive sample cleanup** required (e.g. fractionation, desalting) First sample: $150 Second sample: $75 Third to sixth sample: $40 each (applies to samples submitted together) First sample: $225 Second sample: $112.50 Third to sixth sample: $60 each (applies to samples submitted together)
LC-MS analysis High-performance nanoflow HPLC fractionation of peptides with on-line LC-MS analysis $75 per sample $112.50 per sample
Basic data analysis Protein identification from database search Included with LC-MS analysis Included with LC-MS analysis
Medium complexity data analysis Protein characterization requiring non-automated interpretation of MS and MS/MS data $30 per sample $45 per sample
Medium complexity data analysis Protein quantitation (label-free or using isotopic or isobaric labels), GeLC analyses $55 per hour (with advance estimate) $82.50 per hour (with advance estimate)
MW determination by electrospray ionization mass spectrometry Intact protein molecular weight measurement – includes on-line sample desalting and deconvolution of electrospray data $75 per sample $112.50 per sample

* We have availabe trypsin, chymotrypsin or endoproteinase AspN for protein digestions; other enzymes may require an additional charge, which will be quoted in advance.

** We use ZipTips® and Centricon® centrifugal filter devices for sample desalting; other materials/media may require an additional charge, which will be quoted in advance.


Note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Services are billed at the rate listed on the date of service delivery.