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Biopolymers Laboratory

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Services

DNA Sequencing

Peptide Synthesis

Mass Spectrometry

Protein Digestion for MS & HPLC Mapping

HPLC

Protein Sequencing (Edman)

Charge-back Schedule for MIT Biopolymers Laboratory Services

Service

KI

Non-KI
&
Off-Campus

DNA Sequencing

Individual samples
$5.25 per template
$7.88 per template
96 well plate
$4.20 per template
$6.30 per template

Difficult templates

$7.88 per template
$11.82 per template

Peptide Synthesis - Large Scale (0.1-0.25mmol)
Synthesis
$31.50 per residue
$47.25 per residue
Cleavage, analytical HPLC, MS
$100
$150
Non-standard residues, dyes, etc.
cost of special reagents
cost of special reagents
HPLC Purification
(peak pooling, analytical HPLC, MS)
$200
$300

Peptide Synthesis - Small Scale (5umol)

Synthesis, Cleavage, MS, analytical HPLC

$5 set-up fee/peptide
+
$1.50/residue (≤20 res)
$3/residue (>20 res)

$7.50 set-up fee/peptide
+
$2.25/residue(≤20res)
$4.50/residue (>20 res)
Non-standard residues, dyes, etc.
cost of special reagents
cost of special reagents
HPLC Purification
(peak pooling, analytical HPLC, MS)
$100
$150

Peptide Synthesis - Cellulose Peptide Array (5 nmol)

1 membrane (600 peptides)

$600
$900

Mass Spectrometry - Ion Trap

LTQ MS/MS Protein ID
$50
$75
Enzymatic Digestion (in-gel)
$50
$75

Mass Spectrometry - MALDI-TOF

Analysis
$22
$33
Desalting of Sample
$11
$16.50

Proteomics

Simple sample preparation

(e.g. in-gel or solution protein digestion with standard enzyme*)

Price (Koch Institute)

$100 for first sample, $50 for second sample, $25 each for third, fourth, fifth, sixth sample (applies to samples submitted together)

Price (non-Koch Institute MIT

$150 for first sample, $75 for second sample, $32.50 each for third, fourth, fifth, sixth sample (applies to samples submitted together)

Complex sample preparation**

(e.g. extensive sample cleanup, fractionation or desalting, prior to protein digestion with standard enzyme*)
$150 for first sample, $50 for second sample, $40 each for third, fourth, fifth, sixth sample (applies to samples submitted together)
$225 for first sample, $75 for second sample, $60 each for third, fourth, fifth, sixth sample (applies to samples submitted together)
High-performance nanoflow HPLC fractionation of peptides with on-line LC-MS analysis
$75 per samples
$112.50 per sample
Basic data analysis (e.g. protein identification from database search)
Included with LC-MS analysis
Included with LC-MS analysis
Medium complexity data analysis (e.g. protein characterization requiring  non-automated interpretation of MS and MS/MS data)
$30 per sample
$45 per sample
High complexity data analysis (e.g. quantitation, GeLC analyses)
$55 per hour (advance estimate will be provided)
$82.50 per hour (advance estimate will be provided)
Intact protein molecular weight measurement (includes on-line sample desalting and deconvolution of electrospray data)
$75 per sample
$112.50 per sample

 

*We use trypsin, chymotrypsin and endoproteinase AspN for digestions; if some other enzyme is required there may be an additional charge, which will be quoted in advance.
** We use ZipTips® and Centricon® centrifugal filter devices for sample desalting; if other materials or media are required there may be an additional charge, which will be quoted in advance.

Protein Enzymatic Digestion (NC, PVDF, solution) for HPLC Fractionation

$50
$75

HPLC

Analytical Run
$5
$7.50
Large Scale Purification
(up to 100mg)
$200
$300
Small Scale Purification
(up to 10mg)
$100
$150

Protein Sequencing (Edman)
via Tufts

$50 set-up + $10/cycle
$50 set-up + $10/cycle

Illumina Solexa Genome Analysis

 

 
Single read: approx 5 million 36 base reads

HHMI+ Contibuters $650

Koch Institute: $750

Other MIT: $825

Off Campus: $1,237.50

Paired End a.k.a. Mate Pair reads:

two x approx 5 million 36 base reads

HHMI+ Contibuters $1,100

Koch Institute: $1,200

Other MIT: $1,300

Off Campus: $1,950
MIT logo Copyright © 2007
Massachusetts Institute of Technology 		Biopolymers Laboratory
40 Ames Street  E17-415
Center for Cancer Research
Massachusetts Institute of Technology
Cambridge, MA 02139
(617) 253-7038 		Richard Cook
Lab Director
E17-415
(617) 253-1685
cook@mit.edu
Site last updated July 17, 2008