http://ki/mit.edu/sbc/flowcytometry

Forms and Policies

Home

iLab

Services

Fees

Instrumentation

New Customers

Personnel

Protocols

Forms

What's New

Useful Links

Contact Facility

FAQ

• Analyzer Policy
• Cell Sorting Policy
• Analyzer Training Guidelines  
• Cell Sorting Guidelines
• Publication Policy
• Flow Intro Presentation
• Biohazard Form 

Best Sorting Practices

• Concentrate cells to optimal #’s of 50-100 million cells/ml (Lower concentrations can be run at slower rates)

Lymphocytes-concentrate 50 million cells/ml
Sticky/Adherent cells-concentrate 20 million cells/ml

• Filter your samples using Falcon 12x75mm polystyrene test tubes (Falcon #2235)

• Minimum sample volumes= 0.5 ml

Maximum sample volume= 3.5 ml

• Bring controls
  • Unstained
  • Single color controls

• Bring collection tubes precoated w/100% FBS or 5% BSA. Use 1ml of media + 20% serum or 1ml of 100% serum

• Bring extra collection tubes

• If collecting into plates, add 100 ul of media into wells

How to get cells not to be clumpy for sorting or analysis:

1 mM EDTA sticky cells
10 units DNAse/ml lots of dead cells

For more tips please read our Cell Sorting Guidelines!