Koch Insitute

http://ki/mit.edu/sbc/flowcytometry

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Please refer to this link for the facility's equipment and the flurophores each cytometer can run.

CYTOMETERS                                                         SPECS


FACSCAN'S

Analyzer

Analyzer  FACScan Left: 1 laser, 3 color analyzer with 488 excitation, running CellQuest Pro on Mac OSX. FACScan Right: 1 laser, 3 color analyzer with 488 excitation, running CellQuest Pro on Mac OSX

 

 

FACSCALIBUR'S

Analyzer

 

Analyzer  FACSCalibur Right: 2 laser, 4 color analyzer, running CellQuest Pro on MacOSX

Plate Reader HTS FACSCalibur HTS-1: 2 laser, 4 color analyzer running CellQuest Pro on MacOSX, equipped with HTS (high throughtput sampler), which will load samples from a 96 well plate.

 

FACS LSR'S
Analyzer

Analyzer  FACS LSR II HTS: 4 laser, 10 color analyzer running BD FACSDIVA software on XP, also equipped with HTS. FACS LSR HTS-2:   4 laser, 11 color analyzer running BD FACSDIVA software on XP, also equipped with HTS. LSR FORTESSA HTS: 4 laser, 14 color analyzer running BD FACSDIVA and equipped with HTS.

FACSCANTO

FACSCANTO II

CANTO HTS3 laser, 8 color analyzer, running BD FACSDIVA software on XP, also equipped with HTS.

MOFLO'S

MOFLO

 

 

MOFLO NOZZLE  MoFlo2: 5 laser, 7 color sorter running Summit software on XP. Can sort into 1.5 mL, 5 mL, 15 mL or 50 mL tubes. Up to 2 populations can be sorted at one time. 
MoFlo3: 5 laser, 7 color sorter running Summit software on XP. Can sort into 1.5 mL, 5 mL, 15 mL or 50 mL tubes. Can sort into 96 well plates. Up to 2 populations can be sorted at one time.   

FACS ARIA'S

 

ARIA

ARIA STREAMAria 2: 2 laser, 7 color sorter running BDFACS Diva softwarer on XP. Can sort into 1.5 mL, 5 mL, 15 mL, or 96 well plates. Can sort up to 4 populations.  Aria 3:  4 laser, 11 color sorter running BDFACS Diva software on XP. Can sort into 1.5 mL, 5 mL, 15 mL, or 96 well plates. Can sort up to 4 populations at one time. Aria 1: 4 laser, 11 color sorter running BDFACS Diva software on XP.Can sort into 1.5 mL, 5 mL, 15 mL, or 96 well plates. Can sort up to 4 populations.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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