So Lab - BEAM Bioinstrumentation Engineering Analysis and Microscopy


Research in the So Lab

High-Speed Multiphoton Fluorescence Microscope Based On Polygonal Mirror Scanner


Performance of multiphoton fluorescence microscope is enhanced with the increase of frame rate (0.5 frame/sec to13 frames/sec). It is useful for the following studies:

1) kinetic event such as flow of blood,
2) statistical study of heterogeneous specimen like human skin,
3) biological rare event such as mitotic recombination.

Figure 1: Wide area human skin image. The actual size is 2mm x 2mm. It is the layer of stratum corneum.

Figure 2: Schematic of high-speed multiphoton fluorescence microscope. The polygonal mirror is used for fast x-axis scanning and the galvanometer-driven mirror is used for slow y-axis scanning.

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