So Lab - BEAM Bioinstrumentation Engineering Analysis and Microscopy

  So Lab Facilities

Lasers
  • Nd:YVO4 Laser (Coherent Verdi V10)
     *532 nm
    *10 W average power
    *Single longitudinal mode, line width < 5MHz, Coherence length > meters
    *Power stability 1%
    *Noise 0.04%
    *TEM00
    *Vertical polarization
  • Femtosecond Titanium-Sapphire Laser (Coherent Mira Optima 900F)
    *700-1000 nm
    *2 W CW, 1.5 W Pulsed
    *76 MHz, 150 fs pulse width
    *Power stability 3%
    *Noise 0.1%
    *TEM00
    *Horizontal polarization
  • Spectra-Physics Titanium-Sapphire Laser
    *700-1000 nm
    *80 MHz
    *Power stability < 5%
    *TEM00
    *Vertical polarization

    CW Nd:YAG (Lightwave Electronics, 220 Series)
    *1064 nm
    *7 W
    *TEM00
    *Vertical polarization
  • Frequency Harmonic Generator (Inrad, Model 5-050)
  • *270-330 nm (70-80mW), 350-500 nm (150mW)
Microscopes
  • Multi-Spectral Multi-Photon Microscope
    *Zeiss Axiovert S100-TV
    *Multi-photon scanning capability with Cambridge Technology galvanometric scanner
    *Single photon counting detection
    *Hamamatsu 7400P PMT non-descanned detectors
    *16-channel simultaneous single-photon counting spectral resolved detector
    *Becker-Heckl 730 time correlated single photon counting modulo, 4 channel option
  • Fast Scan Multi-Photon Confocal Microscope
    *Zeiss Axioscope
    *Multi-photon scanning capability with Cambridge Technology galvanometric scanner
    *Video rate imaging capability with Lincoln Laser polygonal scanner
    *Hamamastu 7400P PMT non-descanned detectors for low speed operation, single color channel, single photon counting
    *Hamamastu R3896 PMT non-descanned detector for high speed operation; dual color channel, analog detection
    *Prior stage control for large area imaging
    *Automated microtomy (under development)
  • Micromanipulation Microrheology Microscope
    *Zeiss Axiovert S100-TV
    *Multi-photon scanning capability with Cambridge Technology galvanometric scanner
    *Single photon counting detection
    *Hamamatsu 7400P PMT non-descanned detectors
    *Magnetic Traps (8 poles and single pole) with force up to 10 nN
    *Optical Trap (under development)
    *Fluroescence laser tracking microrehometry (under development)
    *Wide field video rate fluorescence imaging based on Roper Pentmax CCD
    *Objective lauched TIR illumination (under development)
  • Fast Scan Multi-Focal Multi-Photon Microscope (under development)
    *Olympus IX71
    *Multi-focal multi-photon imaging based on multi-anode PMT
    *Single photon counting operation
    *Wide field fluorescence microscope capability
  • Video Fluorescence Microscope
    *Leitz Orthoplan 2
    *UV & 3-color filter set
    *Sony 3 chip CCD camera coupled to video recorder

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