So Lab - BEAM Bioinstrumentation Engineering Analysis and Microscopy
Research in the So Lab
Vibratome for Multiphoton Microscopy
Investigator: Hyuk-Sang Kwon
This project is about making a working prototype of vibratome to fit our current high speed multifocal multiphoton microscopy (MMM) systems.
Vibratome is one of microtomes types with reciprocating knife action for sectioning fresh or lightly treated tissues down to 10 microns with precision of less than 5 micron. The horizontal vibrating blade cutting principle does not distort samples during sectioning. Sectioning may be carried out in a buffer bath which lubricates the blade, prevents heat buildup, facilitates retrieval of the sections and retains important elements of the tissue being studied. For fresh or fixed tissues; not for frozen or embedded tissues. Animal and plant tissues are sectioned without freezing or embedding, thus minimizing artifacts and loss of activity in preparation procedures.
There are known information
using commercially available vibratome. First there are four independent
variables: Forward speed, amplitude, blade angle, bath temperature.
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