Professor Peter So's Laboratory
BEAM group (Bioinstrumentation Engineering And Microanalysis)
{M
}SOLAB FACILITIES
  • Laser Resources
  • (1) Nd:YVO4 Laser (Coherent Verdi V10)
    *532 nm
    *10 W average power
    *Single longitudinal mode, line width < 5MHz, Coherence length > meters
    *Power stability 1%
    *Noise 0.04%
    *TEM00
    *Vertical polarization
  • (2) Femtosecond Titanium-Sapphire Laser (Coherent Mira Optima 900F)
    *700-1000 nm
    *2 W CW, 1.5 W Pulsed
    *76 MHz, 150 fs pulse width
    *Power stability 3%
    *Noise 0.1%
    *TEM00
    *Horizontal polarization

    (3) CW Nd:YAG (Lightwave Electronics, 220 Series)
    *1064 nm
    *7 W
    *TEM00
    *Vertical polarization
  • (4) Melles Griot (Melles Griot, DPSS 58BLDR305)
    *457.5 nm
    *400 mW (in two output beams)
    *Single frequency, line width < 200 kHz, coherence length > meters
    *Elliptical beam
    *Horizontal polarization
  • (5) Argon-Ion (American Laser Corporation)
  • *488-514 nm
  • *8W
    *Polarization (?)
    • (6) Frequency Harmonic Generator (Inrad, Model 5-050)
    • *270-330 nm (70-80mW), 350-500 nm (150mW)

    • Microscope Resources
    • (1) Multi-Spectral Multi-Photon Microscope
      *Zeiss Axiovert S100-TV
      *Multi-photon scanning capability with Cambridge Technology galvanometric scanner
      *Single photon counting detection
      *Hamamatsu 7400P PMT non-descanned detectors
      *16-channel simultaneous single-photon counting spectral resolved detector
      *Becker-Heckl 730 time correlated single photon counting modulo, 4 channel option
    • (2) Fast Scan Multi-Photon Confocal Microscope
      *Zeiss Axioscope
      *Multi-photon scanning capability with Cambridge Technology galvanometric scanner
      *Video rate imaging capability with Lincoln Laser polygonal scanner
      *Hamamastu 7400P PMT non-descanned detectors for low speed operation, single color channel, single photon counting
      *Hamamastu R3896 PMT non-descanned detector for high speed operation; dual color channel, analog detection
      *Prior stage control for large area imaging
      *Automated microtomy (under development)
    • (3) Micromanipulation Microrheology Microscope
      *Zeiss Axiovert S100-TV
      *Multi-photon scanning capability with Cambridge Technology galvanometric scanner
      *Single photon counting detection
      *Hamamatsu 7400P PMT non-descanned detectors
      *Magnetic Traps (8 poles and single pole) with force up to 10 nN
      *Optical Trap (under development)
      *Fluroescence laser tracking microrehometry (under development)
      *Wide field video rate fluorescence imaging based on Roper Pentmax CCD
      *Objective lauched TIR illumination (under development)
    • (4) Fast Scan Multi-Focal Multi-Photon Microscope (under development)
      *Olympus IX71
      *Multi-focal multi-photon imaging based on multi-anode PMT
      *Single photon counting operation
      *Wide field fluorescence microscope capability
    • (5) Video Fluorescence Microscope
      *Leitz Orthoplan 2
      *UV & 3-color filter set
      *Sony 3 chip CCD camera coupled to video recorder

     

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