So Lab's Home Page

Professor Peter So's Laboratory

BEAM group (Bioinstrumentation Engineering And Microanalysis)

High-Speed Multiphoton Fluorescence Microscope Based On Polygonal Mirror Scanner

Investigators: Ki Hean Kim, Peter So

Performance of multiphoton fluorescence microscope is enhanced with the increase of frame rate (0.5 frame/sec to13 frames/sec). It is useful for the following studies:

1) kinetic event such as flow of blood,
2) statistical study of heterogeneous specimen like human skin,
3) biological rare event such as mitotic recombination.

Figure 1: Wide area human skin image. The actual size is 2mm x 2mm. It is the layer of stratum corneum.

It is done by using a spinning polygonal mirror scanner, which can scan faster than the conventional galvanometer driven mirror scanner.

Figure 2: Schematic of high-speed multiphoton fluorescence microscope.

Polygonal mirror is used for fast x-axis scanning and galvanometer-driven mirror is used for slow y-axis scanning.

-----------------------------------[

{Left Me

nu}