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Protein-Carbohydrate Interaction Core (H)

Glycan Array Data

Glycan Array v2.3

Overview

This Core has developed a high-throughput screening platform for identifying lectin-ligand interations. Core H is using a streptavidin/biotin-based glycan array, developed in conjunction with the Carbohydrate Synthesis Core (D), to determine the glycan-binding specificity of glycan-binding proteins (GBPs). A secondary analysis method is being developed to quantitate the relative binding affinities of candidate ligands. This Core is collaborating with the Analytical Glycotechnology Core (C) to isolate, biotinylate, and integrate natural glycans from glycoproteins, cells, and tissues into the glycan array. Information on the protocols used to determine GBP binding specificity and the raw and processed data from the analyses are being deposited into the Central Database.

Specific Objectives

Develop methods for screening the specificity of GBPs
Coordinate with the Carbohydrate Synthesis Core (D) to prioritize preparation of biotinylated oligosaccharides
Establish the binding specificity and affinity of Participating Investigator-supplied GBPs approved by the Steering Committee
Implement a secondary analysis method for quantitation of binding parameters
Implement methods for isolating natural glycans for inclusion in the glycan array

Core H Team

Rick Cummings, Coordinator

Rick Alvarez, Director

Anne Leppänen, Co-Director

Pooi San (Angela) Lee, Analysis of Protein-Carbohydrate Interaction

Thomas K. Johnson, Glycoconjugate Synthesis and Mass Spectrometry

Last Updated Wednesday, 27-Oct-2004 09:48:31 EDT. Please contact us with comments/questions.

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