Information sheet

Readings:

• Lodish 5th ed: Purifying, detecting and characteriing proteins:
  • Chapter 3, p. 86-97; Purification of cells and their parts/visualizing cell architecture:
  • Chapter 5 pages 178-193
• Alberts et al (4th ed.):
  • Chapter 8 (manipulating proteins etc) p.469-491;
  • Chapter 9 (Visualizing cells) p. 547-580

Student comments/questions, lecture 1

Assigned Readings (also for lecture 3, G-Protein signalling):

• Lodish 5th edition:
  • General: Chapter 13
  • Binding properties: p. 533-539
  • Purification: p. 539-541
• Alberts et al (4th ed.):
  • p 160-162
  • General: Chapter 15 (more applicable for later lectures)

Assigned Readings (pdf files):

• Cunningham et al. (dimerization of...)
• Cunningham & Wells (comparison...)
• Kjelsberg et al.

Supplemental Readings (pdf files):

• Biacore
• Calorimetry
• Growth hormone physiology
• Growth hormone in transgenic fish

Background reading
In preparation for this lecture, please review one or both of the following readings to (re)familiarize yourself with the basics of membrane structure.

• Lodish 5th ed: Ch. 5, pp. 147-165 - Membranes;
  pp. 173-177 - Cytoskeleton
  Ch. 18, pp. 743-754 - Lipids
• Alberts 4th ed: Ch. 10 - Membrane structure (covers all three topics together)

In class, we will cover this material very briefly and use it as the basis for discussion of how these facts are established, and to consider them in more depth.

Assigned Readings (pdf files):

• Ervasti & Campbell
• Supplemental figure (Ervasti)
• Ibraghimov-Beskrovnaya et al.

lecture 3 reading resources

Readings:

• Lodish 5th edition:
  • General:  Chapter 13; chapter 13, pages 541-570;
  • chapter 14, pages 598-601;
  • chapter 15, pages 611-621
• Alberts et al (4th ed.):
  • Cell Signaling is found in Chapter 15 (831-906). You should read the whole chapter; however, initially focus on: G Protein-Coupled receptors, p. 852-871. Other aspects of signaling will be covered in subsequent lectures. Endocytic receptors are covered separately in the chapter on Vesicular traffic in the secretory and endocytic pathways (chapter 13, page 746-757). We will cover the material in this chapter later in the course.

Supplemental reading:

• Transduction of Receptor Signals by β-Arrestins by Robert J. Lefkowitz and Sudha K. Shenoy
• Lefkowitz review - arrestin
• Lefkowitz JBC review, 2006 - GPCR traffic (optional)

References and readings, Receptors

Background reading for Lectures 4 – 6  (October 2, 16 and 23).

Please read these before class!

These three lectures, together with their associated discussion sections (October 11, 18 and 25) will cover various types of receptor signaling systems and pathways.

Alberts IV: Chapter 15, especially pages 871-900.
AND/OR
Lodish et al V: Chapter 14

Assigned Readings:

• Kleuss et al. (1991-assignment of...)
• Kleuss et al. (1992-different beta ...)
• Miyawaki et al.

Supplementary readings:

• FRET #1
• FRET #2
• Patch Clamp

Assigned readings:

• Wrana et al. - TGFβ receptor
• Soubeyran et al.

Assigned readings:

• Funamoto et al - Cell 2002
• Dodge-Kafka et al - Nature 2005
• Teruel & Meyer - Cell 2000
• Zhang et al - Nature 2005

Assigned readings:

• Songyang et al.

• to read for discussion in class:
  • Plattner et al.
  • Nash et al.
• for background reading:
  • Nagar et al.

Background reading:

• Alberts: Chapter 19 ( you can omit the section on plant cell walls if you wish)
  OR
• Lodish: Chapter 6.1 – 6.5 ( again, section 6.6 on plant cell walls is optional – but interesting)
• Cell adhesion - Review (pdf file)

Assigned Readings:

• Wojtowicz et al. - Drosophila Dscam
• Lawrence & Springer - Leukocytes

Reading you MUST do before class on Monday Nov 6, 2006:

Chapters discussing Endocytosis (note, we will go over the secretory pathway later in the course)

Lodish: Chapter 17, also chapter 18 p757-767

Alberts: Chapter 13 (focus on endocytosis, but we will cover the secretory pathway later)

• Brown, Goldstein, JBL 1974
• Kirchhausen, Cell 2004
• Kirchhausen, Nature 2004

Reading:

• Townsend et al., Nature, Aug 1989
• Ackerman & Cresswell, Nature Immunology (review), Jul 2004

To read before class:

• Alberts: Chapter 16 and/or
• Lodish: Chapter 19  pp. 779-805.

Background readings:

• Lodish 5th ed:
  • Chapters 7 and 8 (p 313-331, especially ATPases)
    OR
• Alberts 4th ed:
  • Chapter 11 and portions of Chapter 14 focused on ATPase and proton pumping. [Lodish will assume you know material from 'Lodish 5th edition' – at least scan that if you intend to use Alberts]

Readings: (pdf files)

• Gulbis, Mackinnon et al. - Science
• Long, Mackinnon et al. - Crystal Structure - Science 2005
• Long, Mackinnon et al. - Electromechanical Coupling - Science 2005

Supplemental:

• Review of Mackinnon ion channel work, Science Aug 2005

Readings:

• Lodish 5th edition:
  • Chapter 16, Moving proteins into membranes and organelles;
  • Chapter 17, Vesicular Traffic, secretion, and endocytosis -
    You should be familiar with the in vitro golgi transport assay (VSV G protein, fig 17-6), and the SRP-mediated translocation in the ER (fig 16-11)
• Alberts 4th ed:
  • Chapter 12, Intracellular Compartments and Protein Sorting
• Chapter 13, Intracellular Vesicular Traffic

Note: this is a lot of material. You should read all of the suggested reading, but if you don’t have time to do so before class, at least focus on the secretory pathway (ER->Golgi->out), especially the Golgi and glycosylation reactions. We will focus on this! I will assume you are familiar with signal sequences, SRP, SRP receptors and the sec61p translocating pore complexes.

Assigned Readings

• Goldberg & Kornfeld
• Ostermann et al.
• Goldstein et al.
• Brown, Goldstein et al (JBC)

Reading: Cellular Neurobiology 2, Postsynaptic Membranes

• Lodish 5th ed.:
  • Chapter 17, section 17.4

Readings: Cellular Neurobiology 1, Synaptic Release

• Lodish 5th edition:
  • Chapter 17, especially sections 17.2 and 17.6 (other sections will be needed for lectures 11 & 12)
• Alberts 4th ed:
  • Chapter 13 (esp 712-726) [Note: this is a lot of material. You should read all of the suggested reading, but if you don’t have time to do so before class, at least focus on the secretory pathway (ER->Golgi->out), especially the Golgi and glycosylation reactions. We will focus on this! I will assume you are familiar with signal sequences, SRP, SRP receptors and the sec61p translocating pore complexes.]