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Nf1 PCR
Reaction Mix:
Tail DNA |
2.0ul |
10x buffer |
2.0ul |
NII (20uM) |
0.2ul |
NIII (20uM) |
0.2ul |
3'5' (20uM) |
0.4ul |
milliQ |
14.2ul |
Taq (1/25) |
1.0ul |
PCR Primers:
NII |
5' GGT ATT GAA TTG AAG CAC 3' |
NIII |
5’ ATT CGC CAA TGA CAA GAC 3’ |
3'5' |
5’ TTC AAT ACC TGC CCA AGG 3’ |
PCR Conditions:
1. |
94C |
3:00 |
2. |
94C |
1:00 |
3. |
52C |
1:00 |
4. |
72C |
2:00 |
5. |
Goto 2 x33 cycles |
6. |
72C |
5:00 |
7. |
4C |
0:00 |
END |
10X PCR Buffer:
500 mM KCl |
100mM Tris pH 8.3 |
15 mM MgCl2 |
1 mg/ml BSA |
2 mM dNTPs |
dH20 |
(store at -20C)
Expected Bands:
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