pSico and pSicoR Information Page

Adenovirus Cre Intranasal Infection Information Page Protocol

• Protocol
• University of Iowa ordering information

Adeno-Cre or Lenti-Cre Protocol

Dupage M*, Dooley AL*, Jacks T. 2009.
Conditional mouse lung tumor models using adenoviral or lentiviral delivery of Cre recombinase.
Nature protocols, 4(7): 1064-1072.

Genotyping

• Rb
• Rb conditional
• Rb1lox recombined
• p53
• p53 (Berns/ conditional allele)
• p53 (Berns/ conditional allele recombined)
• p53 LSL (reactivatable allele)
• p53 LSL R172H and p53 LSL R270H (conditional) point mutants
• p53 R172H and R270H recombined point mutant alleles (germline)
• p53 Ser23Ala point mutant allele
• p21
• Nf1
• Nf2
• p107
• p130
• p63
• p73
• K-ras LA1/2 PCR
• K-ras LA1/2 Southern
• K-ras KO
• K-ras LSL G12D and 1lox recombined
• H-ras LSL G12D
• N-ras LSL G12D
• RosaCreERT2
• APC flox (exon 14 - Marco Giovaninni's) PDF
• APC flox (exon 1-14)
• miR-17~92
• miR-106a~363 (chromosome X)
• miR-106b~25 (chromosome 5)

DNA Isolation - Proteinase K Method

DNA Isolation - Hot Shot Method

Growing MEFs

Making MEFs