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Seminar on
Modern Optics and Spectroscopy

Wonshik Choi, MIT

"Field-based 3D microscopy for live cell imaging"

March 11, 2008

12:00 noon - 1:00 p.m. Grier Room 34-401


Over the past decades powerful optical microscopy techniques for probing and imaging biological cells and tissues have been developed. Advances have been made to improve spatial and temporal resolution, chemical and molecular specificities, and depth range of imaging. However, most of these developments are based on measuring the intensity of light.  Our group has developed phase microscopy techniques for quantifying not only the intensity but also the phase of the light, which we term as a field-based technique in comparison with conventional intensity-based technique. In this talk, we report a new field-based 3D microscope which can map the distribution of refractive index as well as absorption in live cells and tissues. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will enable quantitative characterization of specimen-induced aberrations in high-resolution microscopy, and have multiple applications in tissue light scattering.

TUESDAYS, 12:00-1:00, GRIER ROOM (34-401)
Refreshments served following the seminar

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Co-sponsored by the George R. Harrison Spectroscopy Laboratory,
the Department of Electrical Engineering and Computer Science and
the School of Science, Massachusetts Institute of Technology.