Light scattering spectroscopy
| Investigators: |
J. Tunnell, S. McGee, J. Mirkovic, R. R. Dasari,
M.S. Feld |
Light scattering spectroscopy (LSS) extracts epithelial nuclear
size distributions from white light reflectance spectra (Perelman
et al. 1998). White light reflectance is collected from the tissue
via a small diameter fiber optic probe (see fastEEM page). Light
that has been singly scattered in the backward direction can be
analyzed to provide the epithelial nuclear size distribution. From
this, several quantitative diagnostic parameters can be computed
including:
- Number density of epithelial nuclei, (#/mm2/um)
- Percentage of enlarged nuclei (%)
- Mean nuclear size, (um)
The figure below shows the extracted nuclear size distributions
for both non-dysplastic and dysplastic tissue. As expected, the
nuclei of the dysplastic tissue exhibit enlarged and more crowded
nuclei as compared to the non-dysplastic tissue.
Recent Publications
- L. T. Perelman, V. Backman, M. B. Wallace, G. Zonios, R. Manoharan,
A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan,
J. Van Dam, J. M. Crawford, and M. S. Feld, "Observation
of periodic fine structure in reflectance from biological tissue:
a new technique for measuring nuclear size distribution,"
Phys. Rev. Lett. 80, 627-630 (1998).
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